Electrochemical Determination of Folic Acid at Sodium Alpha Olefin Sulphonate Modified Carbon Paste Electrode: A Voltammetric Study

TS Sunil Kumar Naik, BE Kumara Swamy^*, CC Vishwanath and Mohan Kumar

Department of PG Studies and Research in Industrial Chemistry, Kuvempu University, JnanaSahyadri, Shankaraghatta, Shivamoga, Karnataka, India

*Corresponding Author:

Kumara Swamy BE
Department of PG Studies and Research in Industrial Chemistry
Kuvempu University, JnanaSahyadri
Shankaraghatta-577451
Shivamoga, Karnataka, India
Tel: +91-8282-256225
Fax: +91-8282-256255
E-mail: kumaraswamy21@yahoo.com

Received date: June 16, 2015; Accepted date: August 22, 2015; Published date: August 29, 2015

Citation: Sunil Kumar Naik TS, Kumara Swamy BE, Vishwanath CC, Kumar M (2015) Electrochemical Determination of Folic Acid at Sodium Alpha Olefin Sulphonate Modified Carbon Paste Electrode: A Voltammetric Study. J Anal Bioanal Tech 6:272. doi:10.4172/2155-9872.1000272

Copyright: © 2015 Sunil Kumar Naik TS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credied.

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Abstract

Sodium alpha olefin sulphonate (SAOS) was used for the modification of carbon paste electrode (CPE) to determine the electrochemical behavior of folic acid (FA) in 0.2M phosphate buffer solution (PBS) at pH 7.4 with the scan rate of 50 mVs-1. The effects of scan rate, concentration and simultaneous determination of FA at modified carbon paste electrode (MCPE) were studied. The effect of interference of dopamine was carried out and real sample analysis of FA was studied at MCPE. From the scan rate and concentration shows that, the overall electrode process was found to be diffusion-controlled at SAOSMCPE and detection limit was found to be 28.8 μM. The modified electrode (SAOSMCPE) exhibits good electrocatalytic activity towards the determination of folic acid when compared to BCPE. The same method can also be applied for other drug analysis.

Keywords

Folic acid; Sodium alpha olefin sulphonate; Modified carbon paste electrode; Cyclic voltammetry

Introduction

Vitamins are a group of organic compounds, essential in small amount for the normal functioning of the body and regulate the metabolic activity [1]. Deficiency of vitamins may result in often painful and potentially harmful diseases. As a result vitamins play an important role in our body. Folic acid (FA) (vitamin BC, vitamin M or vitamin B₉) is a water soluble vitamin and was first discovered in Spinach [2]. During metabolism FA involved in single carbon transfer reactions and it is the precursor of the active tetrahydrofolic acid coenzyme [3]. FA is a nutrient of great importance, especially for women planning for pregnancy. To reduce significantly the incidence and reoccurrence of neural tube defects periconceptual supplementation of folic acid has been demonstrated [4]. Moreover FA is usually employed in the treatment or prevention of megaloblastic anemia during pregnancy [5]. The US Food and Drug Administration introduced mandatory reinforcement of cereal-grain products with folic acid at a concentration of 140 mg/100 g in January 1998 [6]. The Department of Health in the UK proposed fortification of flour with folic acid at 240 mg/100 g [7]. Deficiency of FA gives rise to the macrocytic anemia [8]. Hence FA determination is often required in pharmaceutical, clinical and food samples. There are several methods were reported for the determination of FA including liquid chromatography/tandem mass spectrometry (LC/MS/MS) [9], High Performance Liquid Chromatography (HPLC) [10], Capillary Electrophoresis [11], Microemulsion Electrokinetic Chromatography (MEEKC) [12] and Enzyme linked Immunosorbent assay (ELISAs) [13] and electrochemical methods [14]. Among these techniques electrochemical method is significant one because of its convenience and low cost [15]. FA (Scheme 1) is one of the electroactive species and accordingly we employed electrochemical method for the determination and some of the electrochemical methods for other electro-active species have been reported [16-20].

Scheme 1: Folic Acid.

Dopamine (Scheme 2) is one of the most significant catecholamine, functioning as a neurotransmitter in the central nervous system and deficiency of DA leads to the Parkinson’s disease [21-22]. Changes in DA concentration in biological sample indicate the possibility of abnormalities or diseases in human being. Therefore, the determination of DA plays an important role. DA possesses high electrochemical activity and has been widely studied by electroanalytical techniques to significantly advantage towards biosciences [23-25]. Dopamine has been determined using various electrochemical methods [26]. One of the most significant methods is the determination of analytes by using modified carbon paste electrode through the voltammetic technique, which has the ability to eliminate the interfering substances from DA. The modification can be done by adding different types of modifiers [27-32].

Scheme 2: Dopamine.

In this study, the surfactant (sodium alpha olefin sulphonate (Scheme 3)) is used as a modifier for the electrochemical determination of FA and DA. The term surfactant is derived from surface active agent and is a compound that contains a hydrophilic (attracted to water) and a hydrophobic (repelled by water) segments. Because of their unique molecular structures, surfactant has been extensively used in the fields of electrochemistry and electroanalytical chemistry [33-36] for various purposes. To improve the detection limits of some biomolecules Hu’s group [37-39] has introduced surfactants to electroanalytical chemistry.

Scheme 3: Sodium alpha olefin sulphonate.

In present work, a simple and sensitive voltammetric method was presented for the electrochemical determination of folic acid at SAOS modified carbon paste electrode. The modified electrode showed an excellent electrocatalytic activity for the oxidation of FA in presence of dopamine.

Experimental

Reagents and chemicals

Chemicals used in present work were Folic acid (FA) from Merck chemicals. Stock solution of FA was prepared in 0.1M NaOH. Disodium hydrogen phosphate (Na₂HPO₄), Sodium dihydrogen orthophosphate (NaH2PO4) and Sodium alpha olefin sulphonate were purchased from Himedia chemicals. Spectrally pure graphite powder (particle size <50 mm) from Merck and high viscous paraffin oil (density=0.88 gcm^-3) from Fluka were used for the preparation of the carbon paste electrode. Phosphate buffer (0.2M pH 7.4) was used as optimum measurements. All chemicals used in this experiments were analytical grade and used without any further purification.

Apparatus

Cyclic voltammetric (CV) measurements were performed with a model CHI-660c (CH Instrument-660 electrochemical workstation). All electrochemical experiments were performed in a standard three electrode cell. The bare and sodium alpha olefin sulphonate modified carbon paste electrode (SAOSMCPE) was used as working electrode, Platinum electrode as counter electrode and saturated Calomel electrode (SCE) as the reference electrode. All potentials reported were versus the SCE.

Preparation of bare and modified carbon paste electrode (SAOSMCPE)

The bare CPE was prepared by hand mixing graphite powder and silicone oil in the ratio 70:30 (w/w) for about 30 minutes in an agate mortar to produce a homogeneous mixture. The paste was then packed into the homemade cavity. Sodium alpha olefin sulphonate modified carbon paste electrode (SAOSMCPE) was prepared by immobilizing 15 μL of sodium alpha olefin sulphonate (SAOS) on to the surface of bare carbon paste electrode for 5 minutes.

Results and Discussion

Effect of sodium alpha olefin sulphonate (SAOS) on the surface of CPE

In optimization of modified CPE towards the K₄[Fe(CN)₆], the bare CPE was immobilized on to the surface of the carbon paste electrode in the different concentrations (5, 10, 15, 20, 25 and 30 μL) of SAOS and their electrochemical response towards 1mM K₄[Fe(CN)₆] in presence of 1M KCl as supporting electrolyte was studied. The obtained result illustrates the increase in the quantity of SAOS the corresponding anodic peak current increases upto 15 μL and afterwards the current goes on decreasing (Figure 1), as a result the 15 μL of SAOS immobilized modified CPE was preferred for further electrochemical investigation.

Figure 1: The effect of quantity of SAOS (μL) on the peak current of 1mM K₄[Fe(CN)₆] at the scan rate of 50 mVs^-1 in presence of 1M KCl as supporting electrolyte.

Electrocatalytic behavior of SAOSMCPE at potassium ferrocyanide

In order to standardize the fabricated SAOSMCPE, potassium ferrocyanide was used for the electrochemical parameters. Figure 2 shows the cyclic voltammograms of 1mM K₄[Fe(CN)₆] at bare (dotted line) and SAOSMCPE (solid line) in presence of 1M KCl as supporting electrolyte with a scan rate of 50 mVs^-1. Comparatively SAOSMCPE shows increase in redox peak currents of 1mM K₄[Fe(CN)₆] than BCPE. The probable mechanism is the SAOS surfactant molecules diffuses into the carbon paste electrode along with the potassium ferrocyanide and causes increase in the redox peak signals [40]. The difference in redox peak potential (ΔEp) for BCPE was found to be 0.114 V and 0.084 V for SAOS modified carbon paste electrode. The ΔEp is a function of the rate of electron transfer, hence lower the ΔEp value higher will be the electron transfer rate [41]. The proposed modified electrode shows lower ΔEp value than the BCPE and exhibits great enhancement in the redox peak current. Figure 3a shows the cyclic voltammograms of potassium ferrocyanide at different scan rates. The redox peak current of potassium ferrocyanide goes on increases with increase in scan rate. The plot of Ipa versus scan rate shows good linearity with correlation coefficient value R²=0.99958 as shown in Figure 3b. The plot of Ipa v/s square root of scan rate also shows good linearity with the correlation coefficient value R²=0.9965 as shown in Figure 3c. From the scan rate study the electrode process was found to be both adsorption and diffusion controlled. Based on the above observation SAOSMCPE shows favorable electrocatalytic behavior and it might be used as a chemically modified electrode to explore electroanalytical application.

Figure 2: Cyclic voltammograms of 1mM K₄[Fe(CN)₆] at bare (dotted line) and SAOSMCPE (solid line) in 1M KCl at the scan rate of 50 mVs^-1.

Figure 3a: Cyclic voltammograms obtained for 1mM K₄[Fe(CN)₆] at different scan rates (50 to 150 mVs^-1) containing 1M KCl.

Figure 3b: Graph of anodic peak current (Ipa) v/s scan rate (ν) of 1mM K₄[Fe(CN)₆] in 1M KCl.

Figure 3c: Graph of anodic peak current (Ipa) v/s square root of scan rate (ν^1/2) of 1mM K₄[Fe(CN)₆] in 1M KCl.

Electrochemical response of folic acid at SAOSMCPE

The electrochemical response of folic acid (FA) was studied at SAOS modified carbon paste electrode. The Figure 4 shows the cyclic voltammograms of 0.5 × 10^-4M folic acid at BCPE (dotted line) and SAOSMCPE (solid line) in 0.2M PBS at pH 7.4 with the scan rate of 50 mVs^-1. Comparatively SAOSMCPE shows great enhancement in the anodic peak current (Ipa) than BCPE. Thus remarkable improvement in electrochemical sensitivity towards folic acid at SAOSMCPE gives an evidence for the catalytic effect of proposed electrode.

Figure 4: Cyclic voltammograms of 0.5 × 10^-4 M folic acid at BCPE (dashed line) and SAOSMCPE (solid line) at scan rate 50 mVs^-1 using 0.2M PBS (pH 7.4).

Effect of scan rate on SAOSMCPE

In order to investigate the kinetics of electrode reaction, effect of scan rate (ν) was studied at SAOSMCPE. Figure 5a shows the cyclic voltammograms of 0.5 × 10^-4M folic acid at different scan rate in 0.2M phosphate buffer solution (pH 7.4). The result shows that, with the increase in the scan rate (10-100 mVs^-1) the anodic peak current (Ipa) of folic acid goes on increases. The plot of anodic peak current (Ipa) v/s scan rate (ν) shows good linearity with correlation coefficient value R²=0.99916 as shown in Figure 5b. Along with the plot of anodic peak current (Ipa) versus square root of scan rate (ν^1/2) was also studied and correlation coefficient value was found to be R²=0.9846 indicating the modified electrode process was both adsorption and diffusion controlled electrode reactions (Figure 5c).

Figure 5a: Cyclic voltammograms of 0.5 × 10^-4 M folic acid at SAOSMCPE with different scan rates (10-100 mVs^-1) using 0.2M PBS (pH 7.4).

Figure 5b: Graph of anodic peak current (Ipa) v/s scan rate (ν) of 0.5 × 10^-4 M folic acid in 0.2M PBS (pH 7.4).

Figure 5c: Graph of anodic peak current (Ipa) v/s square root of scan rate (ν^1/2) of 0.5 × 10^-4M folic acid in 0.2M PBS (pH 7.4).

Effect of folic acid concentration at SAOSMCPE

The electrochemical oxidation of folic acid at different concentration using SAOSMCPE was studied. Figure 6a shows the cyclic voltammograms obtained for the folic acid at different concentration in presence of 0.2M PBS (pH 7.4) with the scan rate of 50 mVs^-1. With the increase in the concentration (1.0-3.5 × 10^-4M) the anodic peak current (Ipa) of folic acid goes on increasing. The plot of anodic peak current (Ipa) versus concentration (Figure 6b) shows good linearity with the correlation coefficient value R²=0.9958. The limit of detection (LOD) was calculated by using the formula (1) [42-45] and it was found to be 2.88 × 10^-5M and the limit of quantification (LOQ) was calculated by using the formula (2) and it was found to be 9.6 × 10^-5M.

Figure 6a: Cyclic voltammograms of folic acid at SAOSMCPE with different concentration (1.0, 1.5, 2.0, 2.5, 3.0 and 3.5 × 10^-4M) using 0.2M PBS (pH 7.4) at scan rate 50 mVs^-1.

Figure 6b: Graph of anodic peak current (Ipa) v/s concentration of FA at SAOSMCPE using 0.2M PBS (pH 7.4) at scan rate 50 mVs^-1.

LOD=3S/M    (1)

LOQ=10S/M    (2)

Where, S is the standard deviation and M is the slope

Simultaneous electrochemical determination of FA and DA at SAOSMCPE

The cyclic voltammetry was employed for the simultaneous electrochemical determination of FA and DA. The Figure 7 shows the cyclic voltammograms obtained for the mixture of 0.5 × 10^-4M FA and 0.5 × 10^-4M DA in the presence of 0.2M PBS (pH 7.4) at the scan rate of 50 mVs^-1. The Dotted line shows cyclic voltammograms obtained for the mixture of FA and DA at BCPE. The solid line shows cyclic voltammogram obtained for the mixture of FA and DA at SAOSMCPE. The modified electrode shows great enhancement in peak current (Ip) than BCPE and showing good voltammetric separation for both DA and FA and proved its electrocatalytic behaviour in simultaneous determination. Hence the proposed modified electrode (SAOSMCPE) has the ability for the simultaneous determination of FA and DA.

Figure 7: Cyclic voltammograms obtained for the mixture of FA and DA at bare (dotted line) and SAOSMCPE (solid line) using 0.2M PBS (pH 7.4) at scan rate 50 mVs^-1.

Determination of folic acid in tablet

To investigate the capability of the modified electrode for the determination of the folic acid in the tablet. By using standard addition method, the recovery test was obtained for the voltammetric signals and the observed results were shown in Table 1.

Table 1: Voltammetric signals and their results.

Conclusion

For the electrochemical determination of folic acid, an anionic surfactant sodium alpha olefin sulphonate was used as the modifier. The modified electrode exhibits good electrocatalytic response towards the determination of folic acid. The electrode process of modified electrode was investigated and found to be both adsorption and diffusion controlled and it shows good detection limit. To know the capability of the modified electrode folic acid was determined in the tablet. Finally, it concludes that the proposed electrode (SAOSMCPE) shows very good electrocatalytic properties and can be applied to the electrochemical determination of folic acid.

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