Journal of Pregnancy and Child Health
Open Access

Pregnancy; Iron deficiency anemia; Ferric Sodium EDTA; Folic acid; Iron supplementation;; Safety; Tolerability

Introduction

During pregnancy iron requirement increases for several reasons, including the dilation of red blood cell mass, and iron needs for placenta function and for fetus development and growth. Plasma and blood volumes increase in physiological pregnancies in order to assure proper circulation and oxygen delivery to both maternal body and placenta [1-2]. The World Health Organization (WHO) defines anemia in pregnant women as hemoglobin (Hb) levels less than 11 g/dL, recognizing that the Hb levels in second trimester reach their lowest point [3]. Additionally, the American College of Obstetricians and Gynecologists (ACOG) differentiated by trimester the anemia in pregnancy setting the Hb level of 4].

It has been estimated that the iron amount needed for a normal pregnancy is from 1000 to 1200 mg [1-5]. However, often iron stores of pregnant women are not sufficient to supply these increased requirements and iron absorption from diet can be inadequate, inducing pregnant women to a major risk of occurrence of iron deficiency (ID) and iron deficiency anemia (IDA) [2-5]. In turn, ID and IDA during pregnancy have been associated with major risk of adverse pregnancy outcomes, including preterm delivery, low birth weight, small gestational age (SGA) live birth, preeclampsia, cesarean delivery, maternal and perinatal death [4-6].

Latest estimates showed that anemia affect about 38% of pregnant women globally, with the highest prevalence in South-East Asia regions (48.7%) and Africa (46.3%), followed by Eastern Mediterranean regions (38.9%) and with the lowest incidence in Western Pacific regions (24.3%), the Americas (24.9%) and Europe (25.8%). Therefore, the percentage of anemic pregnant women is still too high, even if several strategies have been applied to stem this disease and global nutrition targets have been defined for improving maternal, infant, and young child nutrition and health [7-8].

For these reasons, WHO guidelines regarding antenatal care for a positive pregnancy experience, issued in 2016 recommend daily supplementation with oral iron (in the dosage from 30 mg to 60 mg of elemental iron) and folic acid (at dosage of 400 μg) for pregnant women to prevent maternal anemia, puerperal sepsis, low birth weight, and preterm birth [7]. In April 2019, the Executive Guideline Steering Group (GSG) updated these guidelines in response to new evidence on two interventions, namely: multiple micronutrient supplements (MMS) and United Nations International Multiple Micronutrient Antenatal Preparation (UNIMMAP), by issuing following recommendation: Antenatal multiple micronutrient supplements that include iron and folic acid are recommended in the context of rigorous research [9]. These guidelines confirm the recommendation for pregnant women that should be supported and encouraged to receive adequate nutrition, achieved through consumption of a healthy and balanced diet [9].

However, the routinely iron supplementation during pregnancy is not always recognized and applied, mainly for the poor tolerability of traditional oral iron therapies that often can cause gastrointestinal side effects and lack of adherence to therapy, and for concerns about safety among women with an adequate iron intake, and about risk of iron overload [10]. A recent Cochrane review evaluated the use of intermittent iron supplementation during pregnancy (two or three times per week) in clinical trials, concluding that this approach is as effective as daily supplementation, decreasing side effects, and probably resulting in higher compliance [10].

New iron sources have been placed on the market with improved efficacy and tolerability, including Ferric Sodium EDTA, consisting of a complex between ferric ion and ethylenediaminetetraacetate (EDTA) representing a highly bioavailable and stable source of iron. The complex between iron and EDTA (NaFeEDTA) is a stable chelate in acid pH conditions and is not being bonded to inhibitors of iron absorption from diet, such as phytic acid or phenolic compounds. The water-soluble chelate once arrived in duodenal tract releases ferric ion, with change of pH, and allows iron to be adsorbed once reduced to ferrous ion trough a duodenum-specific cytochrome b–like protein, Dcytb, a reductase present at the apical surface of enterocytes, as occurs to non-heme iron ingested with diet [11-12].

Ferric Sodium EDTA has been evaluated for treatment of ID and IDA in several studies that was conducted also in pregnant women, children, and adolescents [13-23]. In some study this iron source was added as fortification in whole wheat flour or soy sauce and this measure showed an improvement of hematological and iron status of enrolled subjects [14-20].

Along with iron needs during pregnancy other nutritional deficiencies can occur, like for vitamin C that showed decreased levels in pregnant women, both for increased requirement of mother, that active transfers it to fetus, and for hemodilution. Vitamin C deficiency can be associated with increased risk of infections, premature rupture of membranes, and pre-eclampsia [24]. Also, the role of folic acid supplementation is well recognized, both 2-3 months before and during pregnancy, for the correlation between folate deficiency and neural tube defects (NTD) [24]. Consequently, folic acid supplementation at dosage of 400 μg/day is recommended by WHO guidelines [7].

Other micronutrient’s deficiencies can be harmful during pregnancy, for example, zinc deficiency has been associated with the appearance of pre-eclampsia, postpartum hemorrhage, and even spontaneous abortions, as well as selenium deficiency has been associated with increased risk of miscarriage [24]. For these reasons it is important to have a food supplement that allows to integrate more active ingredients at the same time. One example of Italian product in market is a food supplement based on the association of Ferric Sodium EDTA with vitamin C, folic acid, copper gluconate, zinc gluconate and selenomethionine, named Ferachel Forte^®, where the new iron source has been associated with the other active ingredients to improve iron absorption and erythropoiesis. This product has been already evaluated in several studies and showed efficacy in improvement of hematological status of IDA patients and high tolerability [25-32].

The aim of this study was to verify the effect of the administration of 1 tablet a day of Ferric Sodium EDTA in combination with vitamin C, folic acid, copper gluconate, zinc gluconate and selenomethionine (Ferachel Forte^®), on the hematological status of pregnant women, with reference to the prevention of anemia and iron deficiency in pregnancy.

Materials and Methods

This is a prospective, randomized, open-labelled study conducted on 100 pregnant women visiting the pregnancy clinic of the “Centro Salute Donna”, AUSL Ferrara, of Ferrara city, Italy.

Pregnant women during the first routine visit with over 18 years of age, gestation time 11.0 g/dL on week 12) were recruited. Exclusion criteria were: pregnant women with microcythemia, thalassaemias or other hemoglobin alterations (e.g. sickle cell anemia), pregnant women already with an anemic condition with any etiology, with particular eating habits (like vegetarian - vegan) or with malabsorption (e.g. due to bariatric surgery, celiac disease etc.), women with a history of coagulation disorders.

All enrolled women were randomly allocated to one of following oral treatment groups during entire pregnancy:

• Group A (N=50) treated with Ferric Sodium EDTA in combination with vitamin C, folic acid, copper gluconate, zinc gluconate and selenomethionine (Ferachel Forte^®, AQMA Italia S.p.A, Milan, Italy) 1 tab/day, containing 30 mg of ferric ion, as treatment group

• Group B (N=50) treated with folic acid 1 tab/day, containing 400 mcg of folic acid, as control group.

Simple randomization was performed by using excel random function by matching every numerical ID patient to one of treatment groups. One ID was assigned to every consecutive patient visited in the pregnancy clinic and enrolled in the study. The supplements were administrated every day in the morning. All patients were informed of the study procedures and provided written informed consent. Local ethic boards approved the protocol. The study was conducted in accordance with the Declaration of Helsinki guidelines regarding ethical principles for medical research involving human subjects. Control of blood parameters of Hb, total number of red blood cells (RBCs), ferritin, transferrin was performed at following timepoints: T0 before starting therapy, within the first trimester of pregnancy (

Results

In this study 100 pregnant women were enrolled and randomized to Group A (treatment group) treated with Ferric Sodium EDTA in combination with vitamin C, folic acid, copper gluconate, zinc gluconate and selenomethionine (Ferachel Forte^®) or Group B (control group), treated only with folic acid. Figure 1 shows the flow diagram for the patients enrolled in the study. Enrolled women were 31.7 ± 5.18 years old, at week of gestation < 12, without laboratory indication of IDA. Blood parameters of Hb, RBCs, ferritin, and transferrin were evaluated in 2 Groups of patients and reported in Table 1. Group A did not show statistically significant changes of Hb and transferrin parameters evaluated between T0 and T3. Group A results indicated that pregnant women in treatment with oral iron association maintained almost unchanged blood parameters and the therapy was effective to prevent IDA onset. Group B results showed a worsening statistically significant (P < 0.001) of all parameters evaluated. Blood parameters results reflect the percentage of women developing IDA during the study, according to international ACOG guidelines defining anemia in pregnancy when Hb level is Table 2 shows the percentage of anemic women in both groups. In Group B 84% of women (N=42) became anemic, in comparison to Group A where only 12% of women (N=6) showed IDA.

Table 1: Blood parameters of Group A (treated with Ferric Sodium EDTA in combination with vitamin C, folic acid, copper gluconate, zinc gluconate, and selenomethionine) and Group B (treated only with folic acid).

Abbreviations: SD: standard deviation; RBCs: total number of red blood cells; Hb: hemoglobin; T0: before starting therapy, within the first trimester of pregnancy (<12th week); T1: 20-24 weeks; T2: 30-32 weeks; T3: 36 weeks.

Table 2: Percentage of anemic pregnant women in Group A (treated with Ferric Sodium EDTA in combination with vitamin C, folic acid, copper gluconate, zinc gluconate and selenomethionine) and in Group B (treated only with folic acid).

Figure 1: Flow diagram of patients enrolled in the study. Group A: patients (N=50) treated with Ferric Sodium EDTA in combination with vitamin C, folic acid, copper gluconate, zinc gluconate, and selenomethionine; Group B: patients (N=50) treated only with folic acid.

Regarding the secondary outcome, consisting of the evaluation of tolerability degree of treatment in Group A pregnant women, data are reported in [Table 3] showing that the majority of pregnant women (86%, N=43) considered as good or excellent treatment with Ferric Sodium EDTA association. Overall, about safety outcome the treatment with Ferric Sodium EDTA in combination with vitamin C, folic acid, copper gluconate, zinc gluconate, and selenomethionine was safe and well tolerated, since only 2 women (4%) reported mild adverse events (AEs). These women reported nausea, resolved by assuming treatment after meal. No pregnant woman has discontinued therapy.

Table 3: Tolerability degree of treatment reported by pregnant women in Group A (treated with Ferric Sodium EDTA in combination with vitamin C, folic acid, copper gluconate, zinc gluconate and selenomethionine), evaluated by assigning a score of 0=suspension, 1=poor, 2=fair, 3=good, 4=excellent, at visit T3.

Discussion

This was a preliminary comparative study conducted on 100 pregnant women treated with the new oral iron source Ferric Sodium EDTA, in combination with vitamin C, folic acid, copper gluconate, zinc gluconate, and selenomethionine (Group A) or only with folic acid (Group B), in order to evaluate treatment effect on the hematological status of women and on the prevention of anemia and ID in pregnancy. During pregnancy, ID and IDA can increase the risk of adverse pregnancy outcomes like preterm delivery, low birth weight, SGA live birth, preeclampsia, cesarean delivery, maternal and perinatal death [4- 6]. More in details several studies reported that moderate and severe anemia was associated with higher rates of preterm and SGA live births, along with placental-related morbidity such as preeclampsia, probably caused by placental hypoxia and/or increased oxidative stress.

Furtherly, rates of post-partum hemorrhages, of women undergoing hysterectomy and need of Intensive Care Unit admission, were increased in anemic pregnant women. Also, the percentage of blood transfusions, induction of labor, and cesarean delivery was higher in anemic pregnant women. Mild anemia was associated with higher rates of infectious diseases such as bacterial sepsis [4-6]. Starting pregnancy with adequate iron stores becomes of very high importance for avoiding risk of complications for mothers and babies. Unfortunately, the use of iron supplementation for ID and IDA prophylaxis in pregnancy is not fully recognized, mainly for the low compliance to therapy. Concerns about efficacy and safety of oral iron therapy in gynecologist communities are often derived from an history of use of traditional oral iron therapies, mainly based on low bioavailable iron sources, that can be responsible for gastrointestinal adverse events (AEs) in otherwise healthy pregnant women. Gastrointestinal AEs derive from the amount of iron not absorbed that remains at intestinal level. In addition, the low bioavailability of several oral iron sources, like ferrous sulphate, can be responsible for the low efficacy in improving iron blood parameters [5- 10]. This study aimed to evaluate a new product based on Ferric Sodium EDTA, in combination with vitamin C, folic acid, copper gluconate, zinc gluconate, and selenomethionine, named Ferachel Forte^®, that already showed in previous studies good efficacy and safety profile [25- 32], in pregnant non anemic women to prevent anemia occurrence. This product has been designed to provide a new oral iron with high bioavailability and tolerability profile, since both iron source Ferric Sodium EDTA presents the better characteristics of iron absorption and safety and the other active ingredients in the formulation, such as vitamin C, folic acid, copper, zinc, and selenium, improve furtherly iron absorption and erythropoiesis. In antenatal period not only iron but also other micronutrients deficiencies, like previously reported elements, have been associated with pregnancy complications, like preeclampsia, abnormal neuronal development, postpartum hemorrhage, and risk of miscarriage [24]. Therefore, the use of one single tablet a day providing all active ingredients, useful for the prevention of such deficiencies and fully tolerated by the patients, can be a valid ally for pregnant women. Results of this study showed that in the Group A women treated with Ferric Sodium EDTA, in combination with vitamin C, folic acid, copper gluconate, zinc gluconate, and selenomethionine, blood parameters evaluated remain almost unchanged during the whole pregnancy. In most cases (88%) women arrived at delivery with good hematological parameters and without tolerability troubles. Conversely, most pregnant women of Group B (84%), treated only with folic acid, showed IDA and consequently were excluded from the study in order to start therapy for IDA. This study represents the first one where the use of this specific Ferric Sodium EDTA combination was evaluated in pregnant women and the challenge derived from the consideration that previous studies conducted on this product showed good efficacy and safety results in several anemic patients type, like elderly, frailty, and nephropathic patients [25-32]. Latest results from Giliberti et al. showed that ferric sodium EDTA in combination with vitamin C, folic acid, copper, zinc, and selenium therapy improved not only blood iron parameters but also the inflammatory status of enrolled patients with chronic kidney disease (CKD) [26]. A previous study showed efficacy and safety of Ferric Sodium EDTA association as oral treatment in non-dialysis-dependent CKD elderly patients with secondary anemia not responders to a previous treatment period based on ferrous sulphate [28]. More recently, another study conducted in real-life settings on IDA patients, confirmed efficacy and safety results of this product [25]. Thanks to high tolerability profile of the product, safety issue in pregnant women was not expected, and therefore this preliminary analysis was performed. Dealing with a small sample size, we cannot generalize the results to the larger population of pregnant women, however results are very comfortable and lead us to consider this supplementation a good candidate to start wide prevention campaigns for reduce the onset of anemia and related risks in pregnant women and newborns. Further evaluations will foresee the use of this Ferric Sodium EDTA combination in anemic pregnant women, by setting the adequate dosage according to Hb level and, therefore, severity of anemia in such women. In conclusion, this study confirmed the efficacy and safety of supplementation with Ferric Sodium EDTA in combination with vitamin C, folic acid, copper gluconate, zinc gluconate, and selenomethionine (Ferachel Forte^®), for preventing anemia in pregnant women.

1. Milman N, Taylor CL, Merkel J, Brannon PM (2017) Iron status in pregnant women and women of reproductive age in Europe. Am J Clin Nutr 106: 1655S-62S.

Indexed at, Crossref, Google Scholar

2. Fisher A, Nemeth A (2017) Iron homeostasis during pregnancy. Am J Clin Nutr 106: 1567S-1574S.

Indexed at, Crossref, Google Scholar

3. WHO. Haemoglobin concentrations for the diagnosis of anaemia and assessment of severity. Vitamin and Mineral Nutrition Information System. Geneva, World Health Organization 2011.

Google Scholar

4. Detlefs SE, Jochum MD, Salmanian B, McKinney JR, Aagaard KM, et al. (2022) The impact of response to iron therapy on maternal and neonatal outcomes among pregnant women with anemia. Am J Obstet Gynecol MFM 4: 100569.

Indexed at, Crossref, Google Scholar

5. Georgieff MK, Krebs NF, Cusick SE (2019) The Benefits and Risks of Iron Supplementation in Pregnancy and Childhood. Annu Rev Nutr 39: 121-146.

Indexed at, Crossref, Google Scholar

6. Smith C, Teng F, Branch E, Chu S, Joseph KS, et al. (2019) Maternal and perinatal morbidity and mortality associated with anemia in pregnancy. Obstet Gynecol 134: 1234-44.

Indexed at, Crossref, Google Scholar

7. WHO recommendations on antenatal care for a positive pregnancy experience. 2016. World Health Organization ISBN 9789241549912.

Indexed at, Google Scholar

8. Stevens GA, Finucane MM, De Regil LM, Paciorek CJ, Flaxman SR, et al. (2013) Global, regional, and national trends in haemoglobin concentration and prevalence of total and severe anaemia in children and pregnant and non-pregnant women for 1995–2011: a systematic analysis of population-representative data. Lancet Glob Health 1: e16-25.

Indexed at, Crossref, Google Scholar

9. WHO antenatal care recommendations for a positive pregnancy experience. Nutritional interventions update: Multiple micronutrient supplements during pregnancy. World Health Organization. 2020. ISBN 978-92-4-000778-9.

Indexed at, Google Scholar

10. Peña Rosas JP, De Regil LM, Gomez Malave H, Flores Urrutia MC, Dowswell T, et al. (2015) Intermittent oral iron supplementation during pregnancy. Cochrane Database Syst Rev 10: CD009997.

Indexed at, Crossref, Google Scholar

11. Hurrell RF, Reddy MB, Burri J, Cook JD (2000) An evaluation of EDTA compounds for iron fortification of cereal-based foods. Brit J Nutr 84: 903-910.

Indexed at, Google Scholar

12. Andrews NC (2002) A Genetic View of Iron Homeostasis. Semin Hematol 39: 227-234.

Indexed at, Crossref, Google Scholar

13. Han XX, Sun YY, Ma AG, Yang F, Zhang FZ, et al. (2011) Moderate NaFeEDTA and ferrous sulfate supplementation can improve both hematologic status and oxidative stress in anemic pregnant women. Asia Pac J Clin Nutr 20: 514-520.

Indexed at, Google Scholar

14. Van Thuy P, Berger J, Nakanishi Y, Khan NC, Lynch S, et al. (2005) The use of NaFeEDTA-fortified fish sauce is an effective tool for controlling iron deficiency in women of childbearing age in rural Vietnam. J Nutr 135: 2596-2601.

Indexed at, Crossref, Google Scholar

15. Cignini P, Mangiafico L, Padula F, D’Emidio L, Dugo N, et al. (2015) Supplementation with a dietary multicomponent (Lafergin(®)) based on Ferric Sodium EDTA (Ferrazone(®)): Results of an observational study. J Prenat Med 9: 1-7.

Indexed at, Crossref, Google Scholar

16. Huo J, Sun J, Miao H, Yu B, Yang T, et al. (2002) Therapeutic effects of NaFeEDTA-fortified soy sauce in anaemic children in China. Asia Pac J Clin Nutr 11: 123-127.

Indexed at, Crossref, Google Scholar

17. Herter Aeberli I, Eliancy K, Rathon Y, Loechl CU, Marhône Pierre J, et al. (2017) In Haitian women and pre-school children, iron absorption from wheat flour-based meals fortified with sodium iron EDTA is higher than that from meals fortified with ferrous fumarate, and is not affected by Helicobacter pylori infection in children. Br J Nutr 118: 273-279.

Indexed at, Crossref, Google Scholar

18. Muthayya S, Thankachan P, Hirve S, Amalrajan V, Thomas T, et al. (2012) Iron fortification of whole wheat flour reduces iron deficiency and iron deficiency anemia and increases body iron stores in Indian school-aged children. J Nutr 142: 1997-2003.

Indexed at, Crossref, Google Scholar

19. Longfils P, Monchy D, Weinheimer H, Chavasit V, Nakanishi Y, et al. (2008) A comparative intervention trial on fish sauce fortified with NaFe-EDTA and FeSO4+citrate in iron deficiency anemic school children in Kampot, Cambodia. Asia Pac J Clin Nutr 17: 250-257.

Indexed at, Google Scholar

20. Teshome EM, Otieno W, Terwel SR, Osoti,V, Demir AY, et al. (2017) Comparison of home fortification with two iron formulations among Kenyan children: Rationale and design of a placebo-controlled non-inferiority trial. Contemp Clin Trials Commun 7: 1-10.

Indexed at, Crossref, Google Scholar

21. Huo JS, Yin JY, Sun J, Huang J, Lu ZX, et al. (2015) Effect of NaFeEDTA-Fortified Soy Sauce on Anemia Prevalence in China: A Systematic Review and Meta-analysis of Randomized Controlled Trials. Biomed Environ Sci 28: 788-798.

Indexed at, Crossref, Google Scholar

22. De Regil LM, Jefferds MED, Peña Rosas JP (2017) Point-of-use fortification of foods with micronutrient powders containing iron in children of preschool and school-age. Cochrane Database Syst Rev 11: CD009666.

Indexed at, Crossref, Google Scholar

23. Wang B, Zhan S, Xia Y, Lee L (2008) Effect of sodium iron ethylenediaminetetra-acetate (NaFeEDTA) on haemoglobin and serum ferritin in iron-deficient populations: A systematic review and meta-analysis of randomised and quasi-randomised controlled trials. Br J Nutr 100: 1169-1178.

Indexed at, Crossref, Google Scholar

24. Santander Ballestín S, Giménez Campos MI, Ballestín Ballestín J, Luesma Bartolomé MJ (2021) Is Supplementation with Micronutrients Still Necessary during Pregnancy? A Review. Nutrients 13: 3134.

Indexed at, Crossref, Google Scholar

25. Incarnato A, Curcio A, Paolozzi F, Scialla M, Luongo A, et al. (2022) Evaluation of Oral Therapy Based on Ferric Sodium EDTA, in Combination With Vitamin C, Folic Acid, Copper Gluconate, Zinc Gluconate and Selenomethionine, in Iron-Deficiency Anemia: A Real-Life Study. J Med Healthcare 4: 209.

Crossref, Google Scholar

26. Giliberti A, Curcio A, Marchitto N, Di Lullo L, Paolozzi F, et al. (2022) Comparison of Ferric Sodium EDTA in Combination with Vitamin C, Folic Acid, Copper Gluconate, Zinc Gluconate, and Selenomethionine as Therapeutic Option for Chronic Kidney Disease Patients with Improvement in Inflammatory Status. Nutrients 14: 2116. 

Indexed at, Crossref, Google Scholar

27. Gatti G, Duranti D, Duranti E (2021) Short study on the use of oral Ferric Sodium EDTA in association with vitamin C, folic acid, copper gluconate, zinc gluconate and selenomethionine, in patients with advanced Chronic Kidney Disease. Nephrol Renal Dis 6: 1-3.

Indexed at, Crossref, Google Scholar

28. Di Lullo L, Marchitto N, Curcio A, Romano A, Bilo LL, et al. (2021) The best therapeutic option for oral treatment of secondary anaemia in chronic kidney disease: Role of Ferric Sodium EDTA, in association with Vitamin C, Folic acid, Copper gluconate, Zinc Gluconate and Selenomethionine. Nephrol Renal Dis 6: 1-6.

Indexed at, Crossref, Google Scholar

29. Marchitto N, Curcio A, Iannarelli N, Petrucci A, Romano A, et al. (2020) A pilot study on secondary anaemia in “frailty” patients treated with the Ferric Sodium EDTA in combination with vitamin C, folic acid, copper gluconate, zinc gluconate and selenomethionine: Safety of treatment explored by HRV non-linear analysis as predictive factor of cardiovascular tolerability. Eur Rev Med Pharmacol Sci 24: 7776-7783.

Indexed at, Crossref, Google Scholar

30. Marchitto N, Sindona F, Pannozzi A, Petrucci A, Fusco L, et al. (2019) Role of Ferric Sodium EDTA associated with vitamin C, folic acid, copper gluconate, zinc gluconate and selenomethionine administration in patients with secondary anaemia. Effects on hemoglobin value and cardiovascular risk. Health Sci J 13: 682.

Indexed at, Crossref, Google Scholar

31. Marchitto N, Petrucci A, Fusco L, Curcio A, Romano A, et al. (2019) Effect of Ferric Sodium EDTA administration, in combination with vitamin C, folic acid, copper gluconate, zinc gluconate and selenomethionine, on cardiovascular risk evaluation: Exploration of the HRV frequency domain. Clin Pract 16: 1245-1251.

Indexed at, Crossref, Google Scholar

32. Curcio A, Romano A, Marchitto N, Pironti M, Raimondi G, et al. (2018) Efficacy and Safety of a New Formulation of Ferric Sodium EDTA Associated with Vitamin C, Folic Acid, Copper Gluconate, Zinc Gluconate and Selenomethionine Administration in Patients with Secondary Anaemia. J Blood Lymph 8: 224-225.

Indexed at, Crossref, Google Scholar