Brief Notes on General Analytical Chemistry of Pesticides
Received: 03-Dec-2022 / Manuscript No. jabt-22-83660 / Editor assigned: 05-Dec-2022 / PreQC No. jabt-22-83660 / Reviewed: 19-Dec-2022 / QC No. jabt-22-83660 / Revised: 21-Dec-2022 / Manuscript No. jabt-22-83660 / Accepted Date: 27-Dec-2022 / Published Date: 28-Dec-2022 QI No. / jabt-22-83660
Abstract
Agrochemicals serve critical roles in guaranteeing both food security and food safety. They are essential components in agricultural production and are used to manage or prevent illnesses, insects, and weeds. However, it was impossible to ignore the harm that contemporary synthetic pesticides caused to both people and the environment. To assure the commercial product’s quality and safety for both human and environmental health, the manufacture, registration, application, and monitoring of pesticides should be rigorously regulated. The analytical chemistry used in quality control (QC) testing for commercial formulations, residue analysis, dietary risk assessment, and pesticide regulation prospects is summarised in this paper. The fundamental ideas and components of analytical chemistry for pesticides are thoroughly introduced and examined. The specifications and standards of the pertinent guidelines from various organisations are also covered. The research frontiers and future trends for the development of new generations of agrochemicals, registration and enforcement, market surveillance, and pesticide risk management are finally discussed.
Keywords
Analysis of the formulation; Examination of the residues; Review
Introduction
Pesticides are substances made up of chemically or biologically active elements that prevent, repel, eliminate, or control pests or govern plant growth. They are now primarily referred to as agrochemicals. According to estimates from the Food and Agriculture Organization of the United Nations (FAO), plant pests and diseases annually reduce crop output by 20% to 40% over the world. The contemporary [1] pesticide industry’s development history is depicted in Prior to the 1940s, the majority of those pesticides were natural and inorganic substances, like limestone powder, sulphur, and bordeaux combination. Since the introduction of dichloro-diphenyl trichloroethane (DDT) and hexachlorocyclohexane (HCH) signalled that the pesticide business entered the era of organic synthesis, significant changes have been made in crop protection. One of the most important strategies for managing pests in agricultural production nowadays is the use of pesticides. There are already more than 1500 pesticides on the market worldwide, which are used to reduce crop losses and hence continue to play crucial roles in agriculture, forestry, residential areas, and other areas. However, since the 1960s, there has been a serious concern about the effects of pesticide exposure on humans and other organisms. Pesticide formulation, physical-chemical qualities, and pesticide residues are all included in a wide sense in pesticide analysis. To clarify the composition and quality of pesticide products, pesticide formulation analysis includes qualitative and quantitative examination of the active ingredient and traces of pertinent impurities of pesticide technical material and formulations. The technical material batch analysis, technical material and formulation quality control, and [2] physical and chemical property analysis of pesticide pure substance are all included. Analyzing pesticide residues in agricultural output, food, the environment, and biological samples entails doing both qualitative and quantitative [3-6] analyses. The toxic metabolites of the parent pesticides, their hazardous byproducts, or both, as well as minute amounts of contaminants from chemical reactions or byproducts of degradation during production or storage may all be considered residual indicators. In the three stages of pesticide registration management, comprising pesticides R&D, manufacture, registration, application, monitoring and evaluation, etc. illustrates the necessary scientific experiments or important parts. The analysis of pesticides is crucial to all of the aforementioned factors.
Materials and Methods
Pesticide analysis in agrochemicals discovery and registration
The technical material’s components should be understood during the discovery and registration processes for pesticides in order to further regulate any production impurities and evaluate their nutritional impact. Technical material’s active ingredient, pertinent impurities, and other impurities with a level more than 0.1% are all subject to qualitative and quantitative batch examination. 5 These indicated components’ combined content must range between 98% and 102%. Typically, data generation should involve the analysis of at least 5 typical batches of technical material samples. Relevant impurities are substances that are more harmful than the active component, raise toxicological questions, result in phytotoxicity, or contaminate food products. Additionally, these contaminants may alter formulations’ physical characteristics and leave unfavourable residues in the environment and on food. For instance, anilines, modified anilines, and dimethyl sulphate may be harmful to the environment or human health, or even both. Impurities are mostly produced by by-products of side reactions as well as intermediates formed during the reactions of the raw components. Degradation, isomerization, hydrolysis, oxidation, over-substitution, and rearrangement reactions make up the majority of the side reactions. Iprodione, for instance, may go through ring opening isomerization in both primary and secondary alcohols. Water content and insoluble materials may also function as important contaminants in some formulations, which should be noted in pesticide specifications, according to the Joint Meeting on Pesticide Specifications (JMPS) guideline. 9 In general, nuclear magnetic resonance (NMR), infrared spectroscopy (IR), mass spectrometry (MS), ultraviolet (UV) and X-ray, etc. are used to identify the structure of the contaminants. During the typical batch analysis of technical materials, the composition of the active ingredients and impurities [7- 10] could be clarified. These technical materials could also be subjected to toxicological testing, formulation processing for residue field tests, environmental fate studies, etc. to assist with registration and compliance checking for future market products.
Quality control analysis of pesticide products
In recent years, certain European nations have also mandated the quality and safety evaluation of the adjuvants in the pesticide formulation analysis. Depending on the qualities of the product, different formulations call for different corresponding parameters. For instance, it is necessary to measure the suspension rate, dispersion stability, dry sieving/wet sieving test, dust amount, wear resistance, and mobility, and tablet integrality and disintegration time for suspension concentrate (SC).
Discussion
Physical-chemical properties analysis of pesticide products
Pesticides’ physical and chemical characteristics influence how they behave as residues and how they interact with the environment. These facts could aid in predicting their environmental behaviour and assessing the accuracy and objectivity of data on pesticide residues. Partition coefficient (logKow), hydrolysis, photolysis, melting point, boiling point, solubility, vapour pressure, etc. are a few of the parameters that are frequently measured. Pesticides’ melting, boiling, and solubility points can be used to [9] choose a processing formulation and assess the technical material’s purity. The dispersion and bioaccumulation of pesticides in the environment and animal fat tissues can be inferred using the solubility and partition coefficient, which can also be used to assess the safety of products. 10 According to Zhang et al., average field biota-soil accumulation factor (BSAF) values for organochlorine pesticides with log Kow values of 6.2–7.6 were higher than 1, showing that these chemicals had bioaccumulation in loaches. The metabolism and behaviour of residues in field trials, rotational crop trials, food processing, etc., can be predicted or explained using hydrolysis and photolysis data. When choosing a formulation for a pesticide product, as well as during the manufacture, storage, and transportation procedures, the flash point, explosibility, and flammability all play important roles.
Equivalence of pesticide products
Equivalent products are those whose quality and safety (without higher risk features) are on par with those of the original specification as determined by the examination of the entire package of data. In accordance with FAO JMPS criteria, equivalence is often assessed using a two-tiered methodology. However, when used in actual fields, comparable goods might not guarantee the same efficacy. Equivalence simply indicates that the two items meet the same fundamental quality requirements and have a comparable level of risk.
Pesticide residues analysis
Studies on pesticide metabolism can identify the primary breakdown products and their relationships to occurrence, reaction/ formation, and decomposition, among other things. In order to track and measure the degradation components as far as possible in metabolism investigations, the active ingredient of pesticides should be isotopically labelled. The most commonly used isotopes for studying metabolism include 14C, 31P, 3H, etc.; they can also be used to research food processing and hydrolysis/photolysis. The main method utilised in recent years to determine the chemical composition of the elution peaks is LC-MASS spectroscopy in conjunction with liquid scintillation detection (LSD) detections. Thin layer chromatography (TLC) and offline LSD detection were employed in the early stages of the isotope experiments.
Plant metabolism
Studies on pesticide metabolism can identify the primary breakdown products and their relationships to occurrence, reaction/ formation, and decomposition, among other things. In order to track and measure the degradation components as far as possible in metabolism investigations, the active ingredient of pesticides should be isotopically labelled. The most commonly used isotopes for studying metabolism include 14C, 31P, 3H, etc.; they can also be used to research food processing and hydrolysis/photolysis. The main method utilised in recent years to determine the chemical composition of the elution peaks is LC-MASS spectroscopy in conjunction with liquid scintillation detection (LSD) detections. Thin layer chromatography (TLC) and offline LSD detection were employed in the early stages of the isotope experiments. Higher dosages [6-10] might be necessary to identify the metabolites when crop residue levels are extremely low at the highest advised dose. To define residues and suggest maximum residue limits, the JMPR states that metabolic studies should be reported for each type of at least three of the five representative crop categories (MRLs). In studies on plant metabolism, crops are grouped into five groups: root crops, leafy crops, fruits, pulses and oilseeds, and cereals. A minimum of three sample crops from each of the aforementioned five classes should be the subject of metabolic research in order to extrapolate a pesticide’s metabolic data to all crops. The metabolism studies of the other two groups are not required if the metabolic pathways of the three representative crops are similar.
Metabolism in livestock
Animals that have been exposed to pesticides directly or through their feed may have pesticide residues in their bodies. The major metabolites found in animal tissue and excrement is identified by livestock metabolism studies, and the studies also clarify the metabolic pathway used by pesticides in livestock. These studies provide evidence for residue definitions of pesticides, including whether the metabolites are fat-soluble or not. Animals that are lactating such as goats, cows, and chicks are chosen for investigations on metabolism. It is advised to use one ruminant and 10 chicken each experiment, with administration times of at least five days and seven days, respectively. It is important to measure the total radioactive residue (TRR) in all organs (meat, liver, kidney), excrement, milk, and eggs. Please refer to the testing recommendations, such as the Guidelines for the Testing of Chemicals (No. 503: Metabolism in Livestock), issued by the Organization for Economic Cooperation and Development, for specific instructions on how to conduct experiments (OECD).
Conclusions
An expansion and addition to the GAC concepts now supported in analytical chemistry is the WAC concept put out in this article, together with the RGB 12 algorithm as a specific tool for method evaluation.
Author Contributions
The diagnosis and treatment of this cat were handled exclusively by Jennifer Weng and Harry Cridge. This report was written by Jennifer Weng, and Harry Cridge gave it a critical appraisal. The final draught of the manuscript has received the approval of both Jennifer Weng and Harry Cridge.
Conflict of Interest
According to the authors, there are no conflicts of interest that might be thought to compromise the objectivity of the research presented.
Ethics Statement
The case described in this report was handled as part of the regular clinical caseload at the university teaching hospital; an IACUC or other ethical approval was not necessary. All facets of this patient’s care had the owner’s consent.
References
- Jang KS, Kim YH (2018) Rapid and robust MALDI-TOF MS techniques for microbial identification: a brief overview of their diverse applications. Journal of Microbiology 56:209-216.
- Kim E, Kim J, Choi I, Lee J, Yeo WS, et al. (2020) Organic matrix-free imaging mass spectrometry. BMB reports 53:349.
- Wang Y, Han Y, Hu W, Fu D, Wang G (2020) Analytical strategies for chemical characterization of bio‐oil. Journal of separation science 43: 360-371.
- Ishii K, Zhou M, Uchiyama S (2018) Native mass spectrometry for understanding dynamic protein complex. Biochim Biophys Acta Gen Subj 1862:275-286.
- Takeo E, Sasano R, Shimma S, Bamba T, Fukusaki E, et al. (2017) Solid-phase analytical derivatization for gas-chromatography–mass-spectrometry-based metabolomics. Journal of bioscience and bioengineering 124:700-706.
- Micalizzi G, Vento F, Alibrando F, Donnarumma D, Dugo P, et al. (2021) Cannabis Sativa L.: A comprehensive review on the analytical methodologies for cannabinoids and terpenes characterization. Journal of Chromatography A 1637: 461864.
- Zhu S, Zhao XE, Liu H (2021) Recent advances in chemical derivatization-based chromatography-mass spectrometry methods for analysis of aldehyde biomarkers. Se pu Chinese Journal of Chromatography 39:845-854.
- Grimm R (2021) How Modern Mass Spectrometry Can Solve Ancient Questions: A Multi-Omics Study of the Stomach Content of the Oldest Human Ice Mummy, the 5300-Year-Old Iceman or Oetzi. In Proteomic Profiling 1-12.
- Kuwata K, Itou K, Kotani M, Ohmura T, Naito Y, et al. (2020) DIUTHAME enables matrix‐free mass spectrometry imaging of frozen tissue sections. Rapid Communications in Mass Spectrometry 34:8720-8729.
- Johnson ME, Bennett J, Bustos ARM, Hanna SK, Kolmakov A, et al. (2021) Combining secondary ion mass spectrometry image depth profiling and single particle inductively coupled plasma mass spectrometry to investigate the uptake and biodistribution of gold nanoparticles in Caenorhabditis elegans. Analytica Chimica Acta 1175: 338671.
Indexed at, Google Scholar, Crossref
Indexed at, Google Scholar, Crossref
Indexed at, Google Scholar, Crossref
Indexed at, Google Scholar, Crossref
Indexed at, Google Scholar, Crossref
Indexed at, Google Scholar, Crossref
Indexed at, Google Scholar, Crossref
Indexed at, Google Scholar, Crossref
Indexed at, Google Scholar, Crossref
Citation: Wu Y (2022) Brief Notes on General Analytical Chemistry of Pesticides. J Anal Bioanal Tech 13: 487.
Copyright: © 2022 Wu Y. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Share This Article
Open Access Journals
Article Usage
- Total views: 1068
- [From(publication date): 0-2022 - Aug 17, 2024]
- Breakdown by view type
- HTML page views: 897
- PDF downloads: 171