Industrial Chemistry
Open Access

Numerous industries, including the chemical industry, face substantial issues as a result of fungus contamination. In this industry, fungal proliferation can result in product degradation, lower productivity, and serious health risks. In the context of the chemical industry, this study sought to examine the bioassay and efficacy of micronized fluconazole, a commonly used antifungal drug, against two well-known fungal strains, Candida albicans and Aspergillus niger. In order to assess the inhibitory potential of micronized fluconazole against both Candida albicans and Aspergillus niger, the study used in vitro experiments and microbiological methods [1]. The fungus were at first isolated and identified from tainted samples in the chemical sector. Following the establishment of fungal cultures using standardized procedures, the minimum inhibitory concentration (MIC) method was employed to determine the sensitivity of the cultures to micronized fluconazole.

Fluconazole (C13H12F2N6O)(FNE), a triazole antifungal, is highly bioavailable as determined by micronization, electron microscopy, and bioassay. FNE has a low toxicity and is effective against many pathogenic Candida species. the chemical 1,3-bis(1H-1,2,4-triazol- 1-yl) 2-(2, 4-Difluorophenyl) Propan-2-ol is the IUPAC name for this substance. FNE can be used on the mouth, vagina, throat, and esophagus—the tube connecting the mouth to the stomach—to treat fungal and yeast infections [2]. It can also be utilized to treat organs including the abdomen and lungs. The digestion bundle has FNE completely fascinated. FNE spreads mostly through bodily fluids. The antifungal triazole fluconazole (C13H12F2N6O)(FNE) has a high bioavailability. FNE has a low toxicity and is effective against many pathogenic Candida species. 1,3-bis(1H-1,2,4-triazol-1-yl) is the IUPAC designation for the chemical 2-(2, 4-Difluorophenyl).To treat fungal and yeast infections, FNE can be applied to the mouth, vagina, throat, and esophagus-the tube that connects the mouth to the stomach. In addition, it can be used to treat organs like the lungs and abdomen. FNE is truly enthralled by the digestive package. FNE spreads easily through bodily fluids.

FNE slows down the fungus 14-demethylase Cytochrome P450 enzyme. Compared to mammals, demethylase activity is substantially less susceptible to FNE [3]. This hesitation prevents the conversion of lanosterol to ergosterol, a crucial component of the parasite's cytoplasmic layer, and the subsequent accumulation of 14-methyl sterols. Invasive fungal infections are frequent in critically sick patients, particularly those who have been on mechanical ventilation for a long time. Speed, simplicity, and conservatism are a few useful characteristics that bioassays make up for. Numerous studies support the use of the Candida genus in the FNE bioassay. This study examined the efficacy of micronized FNE in enhancing health safety using bioassays on Candida albicans and Aspergillus niger.

Techniques and materials: Examining the technical active pharmaceutical ingredient sample, which was white, crystalline, and hygroscopic, was the first step in the manufacture of the plain substance. The contaminants in the example total around 0.06%. Methanol, acetone, ethyl acetate, toluene, or any other lingering solvents were not discovered. According to sieve analysis, the FNE has bulk densities of 0.63g/mL (untapped) and 0.76g/mL (tapped). The FNE was filtered using 20 mesh filters. The error was 0.22 percent w/w after drying.

Preparing the material for micronization: The initial step involved loading the untreated material into a dry micronizer apparatus equipped with pressure regulators to manage handling and micronization pressures effectively. Employing moisture-free air, the sample was introduced into the micronizer chamber at a feeding rate of 4 Kg under pneumatic pressure and 6 Kg during micronization. To ensure optimal micronization, this procedure was repeated in three separate iterations [4].

Microorganism procurement and culture maintenance: The fungal strains Candida albicans and Aspergillus niger were procured from GVK Bio, Hyderabad, India, to serve as the test microorganisms.

For prolonged storage, pathogenic strains were cultured on agar slopes with supplemental nutrients at a temperature of 40°C. The slopes were safeguarded using a 25% glycerol solution.

Preparation for the antifungal activity (Agar well diffusion) Preparing antifungal medium: To create the antifungal medium, potato dextrose agar was dissolved in 100 milliliters of distilled water and heated until fully dissolved. The resulting medium was then autoclaved, along with glass petri dishes, at a pressure of 15 Pascal (Pa) for 20 minutes. Under aseptic conditions within a laminar flow chamber, the sterilized petri dishes were filled with the prepared medium.

Discussion and findings: Decreasing particle size and increasing fineness led to an accelerated dissolution rate. Smaller particle sizes also elevated conductivity, surface quality, and product functionality to high standards. Surface modification of micronized FNE particles notably amplified their antifungal activity [5].

Dynamic light scattering (DLS) analysis using Pade Laplace dispersion: The diameter of micronized FNE particles was determined through Pade Laplace dispersion analysis using Dynamic Light Scattering (DLS). This analysis incorporated Rayleigh scattering, Brownian motion, and residual fluorescence. The hydrocolloidal system was transformed into the period-dependent signal using DLS, representing particle decay. Illustrates the Pade Laplace dispersion graph, simplifying the determination of transformed signal components of micronized FNE solution. Dry accessory laser diffraction particle size analyzer evaluated a gram of the sample in the sample holder. Micronized FNE solution was combined with water at a ratio of 1 milliliter to 5 milliliters.

Scanning electron microscope (SEM) examination: SEM was employed to examine the surface morphology and size of micronized FNE at varying magnifications. A drop of micronized FNE solution was air-dried onto a stub and coated with a sputter coater [6-9]. SEM allowed shape discovery at different scan regions through electron beam interaction. Within SEM, an electron beam generated electrons which were subsequently passed through the condenser.

Incorporating these steps and techniques contributed to a comprehensive understanding of the properties and behaviors of micronized FNE, shedding light on its potential applications in antifungal activity and product development.

Agar diffusion: Cylinder-Plate or Cup-Plate Method: Before the assay, dissolved one gram of the fluconazole sample in 0.1N HCl and inoculated it with the required suspensions of Candida albicans and Aspergillus niger in a liquefied medium. After the suspension (sabouraud Dextrose Agar) was added to the media, it was immediately poured into large rectangular plates or Petri dishes with a depth of 3 to 4 millimeters. The thickness of the medium layers is uniform when the dishes or plates are placed on a level surface. Each prepared plate was carefully stored to guarantee that the agar layer surfaces dry when used and that the test organism does not significantly grow or die before the plates are used. Fluconazole is present in the sample at a known concentration of 1 mg/mL. For the two batches of organisms, 001 (normal) and 004 (micronized), separate agar plates with diameters of 3 to 4 millimeters are drilled with a sterile borer. The openings for organic entities are filled with the well-known convergence of the substances 001 and 004 at 1 mg/ml. The solution is poured into each cylinder in the same amount. Under laminar air flow, the plates were kept at room temperature for one to four hours. They were kept for three to five days at temperatures ranging from 20 to 250 degrees Celsius. The circular inhibition's diameter was precisely measured.

Conclusion

Aspergillus fumigatus and C albicans are the two most common fungal pathogens that cause severe invasive infections in immunocompromised patients. FNE is an antifungal with a fluorine substitute that is a bis-triazole. The mechanism of FNE prevents lanosterol from converting to ergosterol by binding to fungal cytochrome P-450 and dislodging fungal membranes as a result. The study found that fluconazole's micronized material is more effective than fluconazole's plain material, which has coarser particles.

1. Brunet R, Boer D, Guillén-Gosálbez G, Jiménez L (2015)Reducing the cost, environmental impact and energy consumption of biofuel processes through heat integration. ChemEng Res Des 93:203-212.

Indexed at, Google Scholar, Crossref

2. Kautto J, Realff MJ, Ragauskas AJ, Kässi T (2014)Economic Analysis of an Organosolv Process for Bioethanol Production. Bio Resources 9:6041-6072.

Indexed at, Google Scholar

3. Nguyen TTH, Kikuchi Y, Noda M, Hirao M (2015)A New Approach for the Design and Assessment of Bio-based Chemical Processes toward Sustainability. Ind Eng Chem Res 54: 5494-5504.

Indexed at, Google Scholar, Crossref

4. Rajendran K, Rajoli S, Teichert O, Taherzadeh MJ (2014) Impacts of retrofitting analysis on first generation ethanol production: process design and techno-economics. Bioprocess BiosystEng 38:389-397

Indexed at, Google Scholar, Crossref

5. Rossetti I, Lasso J, Compagnoni M, Guido G De (2015) H2 Production from Bioethanol and its Use in Fuel-Cells. ChemEng Trans 43:229-234.

Indexed at, Google Scholar, Crossref

6. Rossetti I, Compagnoni M, Torli M (2015) Process simulation and optimisation of H2 production from ethanol steam reforming and its use in fuel cells. 1. Thermodynamic and kinetic analysis. ChemEng J.281:1024-1035.

Indexed at, Google Scholar, Crossref

7. Ren J, Dong L, Sun L, Goodsite ME, Tan S, et al. (2015) Life cycle cost optimization of biofuel supply chains under uncertainties based on interval linear programming. BioresourTechnol187:6-13.

Indexed at, Google Scholar, Crossref

8. Mazzetto F, Simoes-Lucas G, Ortiz-Gutiérrez RA, Manca D, Bezzo F (2015) Impact on the optimal design of bioethanol supply chains by a new European Commission proposal. ChemEng Res Des 93:457-463.

Indexed at, Google Scholar, Crossref

9. Mazzetto F, Ortiz-Gutiérrez RA, Manca D, Bezzo F (2013) Strategic Design of Bioethanol Supply Chains Including Commodity Market Dynamics. IndEngChem Res 52:10305-10316.

Indexed at, Google Scholar, Crossref