Fluorescence Microscopy

"Superoxide radicals were detected in the fungal mycelium by fluorescence microscopy based on dihydroethedium (DE) staining.Fluorescence microscopy technique was also employed for thelive/dead mycelia staining. While staining with 4',6-diamidino-2-phenylindole (DAPI) at 1% revealed all cells (live and dead), the deadcells were selectively detected by propidium iodide (PI) stain.on fungal growth as the mycelia dry weight in the treated cultures was similar to that of the control untreated cultures. On the other hand, at a 25 ppm concentration, the dry weight reduced by more than 50% indicating toxicity. In contrast, pyrene showed a 20% reduction in mycelia mass eve n at 10 ppm concentration, indicating greater sensitivity of the fungus to pyrenein comparison to phenanthrene. OMICS Group International is one of the leading Open Access Publishers which is publishing 700+ peer-reviewed journals with the support of 50,000+ editorial board members as editorial team and aimed to disseminate the scholarly knowledge to the scientific society. OMICS Group also organizing 3000+ International Scientific Conferences and events yearly all over the world with the support of 1000+ Scientific associations worldwide.

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Last date updated on December, 2024