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Volume10, Issue 12 (Suppl)
J Proteomics Bioinform, an open access journal
ISSN: 0974-276X
Page 40
conferenceseries
.com
World Biomarkers & Pharma Biotech 2017
December 07-09, 2017
December 07-09, 2017 | Madrid, Spain
&
20
th
International Conference on
PHARMACEUTICAL BIOTECHNOLOGY
9
th
WORLD BIOMARKERS CONGRESS
JOINT EVENT ON
Antiae a novel biomarker for cytodiagnostics
Elena V Pikuta
1
and
Richard B Hoover
2
1
Athens State University, USA
2
University of Buckingham, UK
A
ntiae is a recently discovered novel external organelle responsible for the gliding motility in bacteria. The antiae composed
of plural thin fibers entangled in plexa that may be 10-20 folds of the cellular size, and spin in a coherent, unison rotation
around the cell cylinder causing a high-sequence trembling that results a smooth slow gliding.
DAPI stain of strain A7P-90mT demonstrated the absorbance of the stain by the antiae that indicates on possible RNA or DNA
molecules involvement in cellular constitution. UV absorbency pick of the living culture was at 300 nm as well as a filtrated
through 0.2 μm filter liquid containing antiae. Here we discuss the results of the proton, sodium pumps and ATPase inhibition
effects on the cellular motion. We also present the results of RNase and DNase treatment along with the thiamine bromide stain
with UV imaging.
We also discuss the chemical composition of the antiae with increased amounts of fluorine and silicon according to X-ray
spectrophotometry. Presence of the antiaewas shown in other bacterial species andArchaea, as well as in viruses. Histopathology
of sarcoma and other malignant cancers demonstrated presence of antiae in cell-free areas affected by cancer tissues that makes
them an important biomarker in cytodiagnostics.
Key words:
bacterial gliding, external organelle antiae, cytodiagnostics, pathohistology, X-ray spectroscopy.
Biography
Elena V Pikuta received her MS in 1995 at Perm State University in Russia, on Biology faculty. She was a practicing nurse in Surgery Department of the Regional
Hospital in Perm for one year.She received her BS degree received in 1984 from Perm Medical College. In University she also defended Diploma in Biophysics
“The Dielectric Parameters of Tissues at Hypoxia”. She was employed by the Institute of Ecology and Genetics of Microorganisms, The Ural Branch of Russian
Academy of Sciences, as a research assistant. She had finished her PhD in 1997 at the Institute of icrobiology of RAS in Moscow, the thesis was “Alkaliphilic
Sulfate-Reducing Bacteria.
epikuta@my.athens.eduElena V Pikuta et al., J Proteomics Bioinform 2017, 10:12(Suppl)
DOI: 10.4172/0974-276X-C1-109