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Volume10, Issue 12 (Suppl)
J Proteomics Bioinform, an open access journal
ISSN: 0974-276X
Page 94
conferenceseries
.com
World Biomarkers & Pharma Biotech 2017
December 07-09, 2017
December 07-09, 2017 | Madrid, Spain
&
20
th
International Conference on
PHARMACEUTICAL BIOTECHNOLOGY
9
th
WORLD BIOMARKERS CONGRESS
JOINT EVENT ON
Ceruloplasmin, lactoferrin and myeloperoxidase in serum of healthy pregnant women
Ewa Skarżyńska
1
, Paulina Wilczyńska
1
, Joanna Żytyńska-Daniluk
2
and
Barbara Lisowska-Myjak
1
1
Medical University of Warsaw, Poland
2
Central Clinical Hospital- Ministry of the Interior, Poland
Introduction&Aim:
Maintaininghomeostasis in terms of redox status determines the suitable environment for thedevelopment
of pregnancy. Metalloproteins: ceruloplasmin (CP), lactoferrin (LF), myeloperoxidase (MPO) modulate oxidative stress. The
aim of the study was to determine the dynamics of changes in the concentrations of these proteins and the relationship between
them.
Methods:
The concentrations of proteins were measured in serum (n=113) in subsequent trimesters, postpartum (n=28) and
in non-pregnant women (n=17) using immunoturbidimetric assay (CP) and ELISA kits (LF, MPO).
Results:
CP [mg/dl] (mean±SD) in trimesters; first (33.0±8.7), second (43.1±6.2), third (44.5±5.8), postpartum (42.39±6.4),
non-pregnant (24.12±7.4) revealed the largest increase between the first and remaining trimesters (approximately 35%). LF
and MPO [μg/ml] (mean±SD respectively) in trimesters; first (6.19±4.54; 0.17±0.12), secend (5.68±4.4; 0.14±0.08), third
(6.34±6.98; 0.17±0.14), postpartum (4.86±3.64; 0.25±0.4), non-pregnant (3.9±2.56; 0.14±0.05) were no significant differences.
Significant correlations were found (p<0.05) between LF vs MPO in all groups as well between CP vs LF and CP vs LF/MPO
ratio in the first trimester and in non-pregnant women respectively.
Conclusions:
CP synthesized in the liver exhibits tends to increase during pregnancy, unlike neutrophil proteins: LF, MPO.
Significant statistical correlations between CP, LF, MPO indicate involvement of these proteins during pregnancy, particular
in the first trimester.
Biography
Ewa Skarżyńska is an university Lecturer and Researcher at Medical University of Warsaw. She received her PhD in Pharmacy Sciences from the same Medical
University. At present she is working on protein biomarkers, particularly involved in maintaining systemic homeostasis including oxidative-reducing equilibrium.
ewaskarzynska@wp.plEwa Skarżyńska et al., J Proteomics Bioinform 2017, 10:12(Suppl)
DOI: 10.4172/0974-276X-C1-110