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.com
Volume 6, Issue 4 (Suppl)
Agrotechnology, an open access journal
ISSN: 2168-9881
Agri 2017
October 02-04, 2017
allied
academies
10
th
International Conference on
AGRICULTURE & HORTICULTURE
October 02-04, 2017 London, UK
Attraction of melon fruit fly,
Bactrocera cucurbitae
(Diptera: Tephritidae) to the odor of cultivable
gut bacterial isolates
Mala Mishra
Indian Agricultural Research Institute, India
T
he melon fruit fly is a destructive pest of cucurbits in India and microbiota plays an important role in insect biology.
Understanding the gut microbiota composition is essential for the development of pest management strategies. Given
this, the study intended to identify the cultivable bacteria inhabiting the intestinal tract of adult melon fruit fly (
Bactrocera
cucurbitae
) from the field-collected population of the experimental fields of IARI, New Delhi, India; between 28°37'22" to
28°39'05" N latitudes and 77°08'45" to 77°10'24" E longitudes. Ten isolates were identified and characterized using biochemical,
molecular and 16sRNA sequencing methods to assess the attractiveness of the bacterial odours to their hosts. Bacteria identified
belong to family
Enterobacteriaceae, Staphylococcaceae, Enterococcaceae, Bacillaceae
and
Brucellaceae
. Laboratory bioassay was
employed to examine the attractiveness of the different bacterial isolates to
B. cucurbitae
adults in normal and olfactory cages
separately. The bioassay showed that a higher number of female flies were attracted to bacterial filtrates than male flies across all
age groups.
Klebsiella oxytoca and Citrobacter freundii
were the most attractive species across all age group of adult flies. These
findings will facilitate blooming of female targeting bacterial bio control emissary for the defense of cucurbit crops.
dr.mala85@gmail.comAgrotechnology 2017, 6:4(Suppl)
DOI: 10.4172/2168-9881-C1-028