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I found sulfo disaccharides which co-working with Klotho. The disaccharides have glucosamine structure and similar structure with hyalunoic acid and chondroitin. Relations of the disaccharide, Klotho, hyaluronic acid, chondroitin, glucosamine with health and anti-ageing were discussed. Klotho makes disaccharide from glucosamin and glucuronic acid and co-works on site with produced disaccharide and gives stable Ca homeostasis and consequent health, anti-aging and long life.