Figure 5: Th1/Th2 ratio determined by cytokine ELISA after F/T stimulation. 3 x 106 cells were distinctly stimulated in vitro with L. major F/T (20 μg/ml). Total Th1/Th2 ratio was verified after F/T stimulation by ELISA determined IFN-γ and IL-5 level. A. Results for DNA-DNA immunization. B. Results for DNA-Live immunization. Each column represents mean of IFN-γ to mean of IL-5 in each group.