Figure 4: Migration and Tube formation assays.
(A) Migration assay: Photographs of HUVEC cells from the underside
of Boyden chamber membrane. Number of cells migrated
in ICM (incomplete medium) used as negative control. Number
of migrated cells in ICM with 25 ng/ml bFGF or with bFGF and 1μM tumstatin or 1 μM a1(IV)NC1 were viewed using Olympus
CK2 light microscope and representative fields (200x magnification)
are shown. (B) Migration assessment assay: The graph
displays average number of cells migrated in three independent
experiments. (C) Tube formation assay: Tube formation was
evaluated after 24 hrs on Matrigel™ matrix with and without 1μM tumstatin or with 1 μM a1(IV)NC1 proteins. Tube formation
was visualized in Olympus CK2 light microscope and representative
fields at 100x magnification are shown. (D) Tube formation
assessment: The graph displays average number of tubes in
three independent experiments. In A and C, arrows indicate migrated
endothelial cells and tube formation respectively. |