Figure 4: Evolution of hydrogen concentrations (left) and rates of H2 production (right) using optimal values of the parameters that were optimized by single or double stage optimization protocols. The parameters that were optimized by single stage optimization are enzyme initial concentration (E0), enzyme degradation parameter (λ), enzyme substrate affinity (Km) and enzyme turn-over (kcat). The combination of parameters that were optimized by double stage optimization was E0+λ and E0+Km. Simulations time were of 9000 min with 70 mM cellobiose, 70 mM of phosphate and 1mM of NADP as starting substrate concentrations.