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| Figure 1: CRISPR-Cas9 target recognition. In the CRISPR system, a sgRNA (containing a CRISPR RNA (crRNA) and a partially complementary transactivating RNA (tracrRNA) is essential for RNA processing and for recognition by Cas9 (CRISPR- associated protein 9). Cas9 is a RNA-guided, dsDNA binding protein that uses a nuclease to cleave both strands of target DNA. Cas9 relies on the PAM site and base pairing with the sgRNA and the target DNA. |
