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| Figure 1: Transfection of macrophages with the eIF2α-S51A mutant attenuated E‾/B48 lipoprotein-induced activation of the eIF2α�ATF4 signaling pathway. Raw 264.7 cells were stably transfected with a plasmid vector expressing eIF2α- S51A (S51A) or an empty pEGFP vector. The cells were incubated at 37�C for 24 hrs with 20 µg/ml of E+/B48 or E‾/B48 lipoproteins or culture medium alone (control). The levels of total eIF-2α and ATF4 proteins and the levels of phosphorylated eIF-2α (eIF2α-p) were determined by western blot analysis and expressed relative to β-tubulin levels. Values represent the mean � SEM of five separate experiments. *P<0.05 as compared with the control, and �P<0.05 as compared with cells transfected with empty pEGFP vector and treated with E‾/ B48 lipoproteins. |
