Figure 5: A: ELISA with purified scFv. Antigen: 1-GBV-C-NS3, control-GBV-C-NS5B, 2-antigen: GBV-C-NS4A/B, control-GBVC- NS5B, 3-6-antigen: GBV-C-NS5B, control GBV-C-NS3. Recombinant antibodies: 1-scFv-9, 2-scFv-9, 3-scFv-17, 4-scFv-22, 5-scFv-33, 6-scFv-34. B: Dot Blot assay. A1-antigen: GBV-C-NS5B, antibodies: anti-GST; A2-antigen: GBV-C-NS5B, antibodies: scFv-33; A3-antigen: scFv-33, antibodies: anti-His; A4-antigen: GST, antibodies: scFv-33; B1-antigen: GBV-C-NS5B, antibodies: anti-GST; B2-antigen: GBV-C-NS5B, antibodies: scFv-34; B3- antigen: scFv-34, antibodies: anti-His, B4-antigen: GST, antibodies: scFv-34. |