Figure 1: Cyclosporine A (CsA) effect on GFP and Cytokine gene expression.. (A) IL-2; IFN-γ:,IL-4; and IL-10 reporter jurkat cell lines were treated with CsA activated by PMA/Ionomycin (B) TNF-α reporter THP-1 cell line was treated in presence of LPS at IC-10 (10μg/ml) and two concentration of IC10/10 and IC10/100 for 48 h. Relative fold change in expression of GFP from these cell lines was calculated in comparison to expression of GFP in untreated control reporter cells. ) (C) IL-2; IFN-γ:,IL-4; and IL-10 reporter jurkat cell lines were treated with CsA activated by PMA/Ionomycin (D) TNF-α reporter THP-1 cell line was treated in presence of LPS at IC-10 (10μg/ml) and two concentration of IC10/10 and IC10/100 for 24 h. Real time PCR was done on RNA extracted from these treated cells. Relative fold change in expression of cytokine`s gene was calculated in comparison to their expression in untreated control cell. Results are shown as mean fold change ± SEM (n=3) and change was considered significant at P ≤ 0.05 (*) and P ≤ 0.01 (**).