Figure 4: Extracted cells internalize and transport the vital dye FM to the vacuole membrane. Wild-type cells were grown in YPKG for 3 days and divided. Half of the cells were subjected to the extraction procedure, while the other half was not extracted. Non-extracted and extracted cells were then treated with or without Triton X-100 (TX) for 30 min. Cells were centrifuged and resuspended in YP containing 1 μl FM (16 mg/ml) for 3 hours. The distribution of FM in non-extracted cells (A), non-extracted cells treated with TX (B), extracted cells (C), and extracted cells treated with TX (D) was observed by a Zeiss microscope. |