Figure 2: Changes in levels of Rac1 and PLC-γ1 proteins, Rac1/ PLC-γ1 complex, [Ca2+]cyt, and cell migration in the presence or absence of cellular polyamines. A: representative immunoblots of Western analysis for Rac1 and PLC-γ1 proteins. Differentiated IEC-Cdx2L1 cells were grown in the DMEM media containing 5 mM α-difluoromethylornithine (DFMO) alone or DFMO plus 10 μM putrescine (Put) for 4 and 6 days. Levels of Rac1 and PLC-γ1 proteins were measured by Western blot analysis. B: levels of Rac1 and PLC-γ1 proteins in the complex immunoprecipitated (IP) by the anti-PLC-γ1 antibody from cells exposed to DFMO or DFMO + Put for 4 days. C:summarized data showing resting [Ca2+]cyt concentrations (left) and the amplitude of CPA-induced Ca2+ influx (right) from cells described in B. Values were means ± SE; n = 20. *P < 0.05 compared with control cells and cells exposed to DFMO plus Put. D: summarized data showing cell migration 6 h after wounding in cells described in A. Values are means ± SE of data from 6 dishes. *P < 0.05 compared with control cells or cells treated with DFMO plus Put.