Figure 1: Schematic representation of deconstructed version of plant virus expression vectors.
a). deconstructed TMV expression vector for production of active replicons in plants. Virus replicase, MP: movement protein, CP; coat protein, RS: recombination site. 35S: CaMV 35S promoter, Nos: nopaline synthetase terminator.
b). CPMV RNA-2 nonreplicating construct for high expression of vaccine proteins. This is the RNA that is inoculated into plants for transient gene expression. NTR: nontranslated regions, 512: nucleotide position of CPMV RNA-2 first open reading frame, fused to gene of interest.
c). Deconstructed BeYDV expression vector. Rep; replication initiator protein, LIR: long intergenic region, SIR: short intergenic region.