Figure 3: (A) The mRNA of PITX2、LEF-1 and β-catenin all decreased respectively as compared with their scrambled control, when added the inhibitor of DKK1. (B) Western blot analysis using anti-PITX2 antibody, anti-LEF-1 antibody and anti-β-catenin antibody showed the reduced expressions of PITX2、LEF-1 and β-catenin by the inhibitor of DKK1.