Figure 3: Demonstration of the blastic plasmacytoid dendritic cell neoplasm immunophenotype on flow cytometry. The CD45/SSC blast gate (blue cells in top row, left) is further gated for CD19(-) CD3(-) cells (top row, middle). The remaining cells are gated for CD4(+) CD56(+) (top row, right). The BPDCN population is located within the CD4(+) CD123(+) group of cells (bottom row, left) and the CD56(+) CD123(+) group of cells (bottom row, middle). They are negative for myeloperoxidase (MPO) as shown at the bottom right of the figure. |