Figure 7: Expression of IL-10 and IL-2 in Jurkat cells. Jurkat cells cultured in pH 7.6 and 6.3 media for 24 h were treated with OKT-3 (0.2 μg/ml) or CD28.6 (5 μg/ml) for 5 min. Whole RNA was extracted and mRNA levels of IL-10 (A) and IL-2 (B) were measured with real-time PCR as described in Materials and Methods. The means and standard deviations of six measurements obtained from cells of two independent cultures are represented. Statistical analysis of IL-2 expression: p<0.01 compared with “no addition” at pH 7.6; p>0.3 compared with “no addition” at pH 6.3. |