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| Figure 3:Western blot and densitometry analysis of OCT-4 and PLZF in 5, 10, 20 and 35 dpp mice. Expression levels of OCT-4 protein in the testes of 5, 10, 20 and 35 dpp mice by Western blot (a) (i = OCT-4 +ve, ii = -ve control for OCT-4, iii = GAPDH + ve, iv = -ve control GAPDH) and densitometry analysis Expression levels of PLZF protein in the testes of 5, 10, 20 and 35 dpp mice by Western blot (b) (v = PLZF +ve, vi = -ve control for PLZF, vii = GAPDH + ve, viii = -ve control for GAPDH) and densitometry analysis (c) (** indicate statistically significant differences compared with 5 dpp expression p<0.01, *** indicate statistically significant differences compared with 10 dpp expression p<0.001) (d) (*** indicate statistically significant differences compared with 5 and 10 dpp expression p<0.001). Mean ± SD, n = 5 mice per age group |
