Figure 3: Thy-1 is Highly Expressed in Human Gastric Cancer-associated Fibroblast (CAF). (A) Representative immunohistochemistry staining of Thy-1 expression in gastric cancer samples. a. Thy-1 was predominantly presented in the cytoplasm and membrane of the spindle-shaped cells among the nests of gastric carcinoma, i.e. gastric CAF cells as indicated by arrows (ADD ARROWS IN THE FIGURE). b. Normal gastric tissue has low expression of Thy-1. Scale bar: 100 μm. Magnification: 100× (BETTER TO INSERT A IMAGE WITH HIGH MAGNIFICATION). (B) Thy-1 expression in CAF clones and paired NF clones isolated from 5 gastric specimens. Note that in these 5 CAF clones, 4 CAF clones (CAF01, CAF02, CAF04 and CAF05) showed significant increase of Thy-1 protein expression relative to the paired NF clones, as indicated by densitometry analysis. Only 1 CAF clone (CAF03) expressed a similar level of Thy-1 to that observed in the paired NF clone (NF03). ***, p<0.001, relative to paired NF clones. (C) Real-time PCR analysis for Thy-1 mRNA expression. Similar to the results from the Western blot analysis, Thy-1 mRNA level was significantly up-regulated in 4 CAF clones (CAF01, CAF02, CAF04 and CAF05) compared to the paired NF clones Only the CAF03 clone expressed a similar level of Thy-1 to that observed in the paired NF03 clone. ***, p<0.001, relative to paired NF clones.