Figure 1

Figure 1: Enumeration of blood MDC and PDC. (A) Gating strategy for defining multiple blood MDC subsets and PDC. Left dot plot: events shown are gated upon CD45⁺ WBC. Middle dot plot: total CD11c⁺MDC (Gate 2) and CD123⁺PDC (Gate 3) are gated upon Lin^-HLA-DR⁺ cells (Gate 1). Right dot plot: CD16^-CD141⁺MDC (Gate 4), CD16⁺Cd141^-MDC (Gate 5) and CD16^-CD141^-MDC (Gate 6) are gated upon CD11c⁺MDC (Gate 2). (B) Scatter plot of the absolute number of total CD11c⁺MDC (median), CD16⁺MDC (median), CD16^-CD141^-MDC (median), CD16^-CD141⁺MDC (mean) and CD123⁺PDC (mean). Absolute numbers of CD16⁺MDC and CD123⁺PDC correlated with age in T1D children, siblings and controls (Spearman P and r value). *, P < 0.05; **, P < 0.01.