Figure 3: CTL expanded in ZYX Bioreactor leads more Annexin V expression of target cells. Annexin V banding assay were conducted with flowcytomety. It was assessed by the percentage of Annexin V positive cells/CD3-CD20-CD33- cells, in which the apoptosis occurred in the culture with the T cells not stimulated by cancer cells as background was subtracted. The CTL expanded in ZYX Bioreactor induced more Annexin V expression of target cells in comparison with static culture at all E:T ratios (P<0.01, n=6).