Figure 5: Cytokine production in polarized macrophages following Poly I:C stimulation. MDMs were polarized as described previously. Polarizing stimuli were removed after the 2 days and polarized macrophages were treated with Poly I:C (50 μg/ml) for 24 h. Cytokine levels in the supernatants were measured using flow cytometry as described in Materials and Methods. Bar graphs represent mean ± SEM, *p ≤ 0.05; **p ≤ 0.005; ***p ≤ 0.0005 with at least n=5.