Figure 1: Susceptibility of human M1 and M2 macrophages to Influenza viruses. (A) CD14+-cells were purified from human PBMCs cultured in presence of 5% autologous serum, recombinant GM-CSF, GM-CSF+IFNγ, M-CSF, M-CSF+IL4 or M-CSF+IL10. Cells were incubated with Influenza A/California/04/2009 virus (H1N1), A/Quail/Hong Kong/G1/97 (H9N2) or A/Hong Kong/483/97 (H5N1) at Multiplicity of Infection (MOI) of 2. At 3, 6 and 24h p.i. cells were fixed with 4% paraformaldehyde, permeabilized and stained for influenza A nucleoprotein and DAPI. The chart showed the median of three different experiments from 3 independent donors. (B) Table shows statistical significance of % of influenza infection of the different macrophages at each time point *p<0.05, **p<0.01, ***p<0.001. Red shows that the reference cell line (listed at the top of column) is significantly higher than the comparator (cells listed on the left) and black shows that it is differently lower, using two-way ANOVA followed by a Bonferroni multiple-comparison test.