Figure 1: FACS analysis of NKT cell populations with or without P. pneumotropica infection. (A) Representative FACS plot showing the percentage of NK1.1+ and CD4+ NKT cells in the different groups as shown in (B). Percentage of (B) CD4+ NKT cells and (C) CD8+ NKT cells in gated CD3+ T cells in analyzed splenocytes and (D) DN NKT (CD3+CD4-CD8-NK1.1+) cells for all groups of mice (non-infected WT and CD1d-/- mice; and infected WT and CD1d-/-). Dot plots (B–D) show individual values and the line represents the mean. One-way ANOVA test with Newman-Keuls multiple comparisons correction was used to evaluate significant differences among the groups; n = 6 mice/group, **P =0.01, ***P =0.001.