Figure 1: Morphological characterization of S. aurata primary neuronal cells. (a) Morphology of freshly isolated staminal neural cells (CNS) with the characteristic ovoid shape (a’, a’’), 1 day after seeding (×200). (b, b’, b’’) Early elongated attached glioblast progenitor after 2 days (×200). (b’’) Negative inverted phase contrast image showing newly formed glioblasts from neuron precursor cells (NPC) in 6 day old culture (×200). (c) Glioblast (c’) Neuroblast (d) Morphology of Neuron Precursor Cells (NPC) which generate neurons but not glia: here is visualized a colony showing new synthetized neurons (×200). (e, e’) Morphology of neuronal Progenitor showing a cluster of newly synthetized neural cells, 4 day old culture (×200). (f) Morphology of a bipolar neuron after 2 day (f) and pseudo-unipolar neuron after 2 weeks of culture (f’) showing the characteristic soma ovoid shape. (g) Bipolar shaped neuroblasts immunolabeled with GFAP-FITC (green, g’), with beta III tubulin-TRICT (red, g’’’), merged image of g’ and g’’’ (g’’). Scale bar = 50 μm (a); 20 μm (f); 10 μm all.