Figure 1: Agarose gel electrophoresis of PCR products, lane M=DNA ladder, lane 1=negative control, lane 2=positive control (Vp. ATCC17802), lane 9, 10, 12, 13, 14, 15, 17, 18=negative results, lane 3, 4, 5, 6, 7, 8, 11, 16=toxR positive at band 368bp, lane 6, 7=tdh & trh positive at bands 251bp and 484bp, respectively. |