Figure 4: (A) The mRNA and protein expression levels of MMP-2 in the five groups and the effects of Yellow wine on mRNA expression of MMP-2 stimulated by Hcy (100 μmol/L) in cultured VECs (48 h). The mRNA of MMP-2 was tested by Real-time PCR (Figure 4A), and MMP-2 protein was examined by western blot assay. (B) A: x ±s, n=9, *P<0.01 vs. N (control); ##P<0.01 vs. Hcy; and P< 0.01 vs. Ethanol B: x ±s, n=5, *P<0.01 vs. control; **P<0.05 vs. Hcy; ##P<0.05 vs. Hcy+Ethanol.