Figure 2: Expression levels of the indicated genes in the oprF mutant H636, relatively to P. aeruginosa H103. mRNAs were assayed by quantitative RT-PCR performed on RNA extracted from H636 and H103 strains. Values above and below 1 show a higher and a lower mRNA level in H636 than in H103, respectively. PCR reactions were performed in triplicate and the standard deviations were lower than 0.15 CT. The experiments were performed twice with independent bacterial cultures.