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| Figure 5: Effects of PMMA on Monoamine Oxidase (MAO) activity in the mouse limbic forebrain. (A) For total MAO activity (MAO-AB) assay, each reaction used 1 mM p-tyramine as the substrate. After the PMMA (0.04 -4 mM) treatment, samples were incubated for 60 min. (B) For the MAO-B activity assay, each reaction used 1 mM benzylamine as the substrate. After the PMMA (0.04 -4 mM) treatment, samples were incubated for 60 min. Each column represents the mean with the S.E.M. of 6-7 animals. **P<0.01 vs. the vehicle (Veh)-treated group. (C) Effects of selective MAO-B inhibitor, pargyline, on monoamine oxidase (MAO) activity in the mouse limbic forebrain. Each reaction used 1 mM p-tyramine as the substrate; after the pargyline (Parg, 1 μM) treatment, samples were incubated for 60 min. For MAO-A activity assay, each reaction included the MAO-B inhibitor, pargyline (Parg, 1 μM), with 1 mM p-tyramine as the substrate; after the PMMA (0.4 mM) treatment, samples were incubated for 60 min. Each column represents the mean with the S.E.M. of 9 animals. **P<0.01 vs. vehicle (Veh)-treated group. ##P<0.01 vs. pargyline (Parg)-pretreated group. |
