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Controlled Expansion of Mammalian Cell Populations by Reversible Immortalization
In 1996, reversible immortalization using SV40 large T antigens and the Cre/LoxP system was successfully achieved with primary human fibroblasts. The concept of reversible immortalization involves introducing an immortalizing agent, SV40 large T antigens, into primary cells, expanding the cells in the culture, and finally, effic.. Read More
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The History of Modern Biotechnology in Iran: A Medical Review
The origin of biotechnology goes back to millenniums ago. The ancient China and Egypt were the first countries that used biotechnology in the form of food fermentation. The modern biotechnology especially in the field of medicine is introduced as “Technology of Hope” as many people are favored from the medical biotec.. Read More
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The pathogenesis of many neurodegenerative diseases, including Alzheimer’s disease (AD) is characterized by protein aggregation into amyloid fibrils. In AD, the fibrils are of the amyloid-β (Aβ) peptide. The development of new approaches based on nanotechnology for early detection and potential treatment of AD is.. Read More
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Abstract Background: Omega-3-fatty acids are essential fatty acids necessary for various human health aspects. Humans can get Alpha-linolenic Acid (ALA) from plants and can convert to LCPUFAs, but to very less extent. LCPUFAs can be obtained only from fish oil which is not acceptable by vegetarian population. Hence, there is .. Read More
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Microbial Synthesis of Silver Nanoparticles: A Review
This review paper proposes the synthesis of nanoparticles from microbes and its applications. Several microorganisms like E. coli, Lactobacillus, Pseudomonas, etc. are involved in the nanoparticles synthesis such as gold, silver, titanium, etc. This is a part of the microbes’ internal metabolism. The microorganism used for.. Read More
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Significance of Biotransformation in Drug Discovery and Development
Biotransformation is a process by which organic compounds are transformed from one form to another, aided by organisms such as bacteria, fungi and enzymes. Biotransformations are used as a valuable strategy to build molecules, similar to parent drug in the drug discovery programme. Biotransformations can also be used to synthesi.. Read More
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Breeding of Sistan Yaghouti Grape using Clonal Selection
Sistan Yaghouti grape belongs to the category of seedless grapes which are resistant to diseases and they have an uninterrupted growth in adverse conditions. Sistan weather usually has hot wind and salty soil. In order to increase the fruit quality, we investigated vine yards of Sistan areas such as Zahak, Dust-Mohammed and Bonj.. Read More
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In vivo Efficacy of PAMAM-Dendrimer-Cisplatin Complexes in SKOV-3 Xenografted Balb/C Nude Mice
Loco-regional delivery of cisplatin by Intraperitoneal (IP) administration in ovarian cancer is highly advantageous, since it exposes high concentrations of cisplatin to tumors in the peritoneal cavity. PAMAM-dendrimer-cisplatin complexes are expected to enhance the penetration of tumors in the peritoneal cavity, and aid in enha.. Read More
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Growth Kinetics of Acclimated Mixed Culture for Degradation of Isopropyl Alcohol (IPA)
Iso-propyl alcohol (IPA) is an organic chemical regarded as a potential pollutant. The permissible exposure limit (PEL) of IPA specified by Occupational Safety and Health Administration (OSHA) is 400 mg L-1. In this study the aerobic biodegradation of IPA is carried out by an acclimated mixed culture obtained by sewage treatment.. Read More
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Stereospermum suaveolens (DC.) is a multipurpose tree endowed with medicinal properties and good timber wood quality. It has analgesic and wound healing property. It is one of the important tree species used in the preparation of ayurvedic formulation known as Dashmula, in which roots of Dashmula tree species are used in prepara.. Read More
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Seven differentially-expressed cDNA clones were isolated by using an mRNA differential display between a Dictyostelium wild-type AX2 and a mutant HG794 defective in O-glycosylation. Transcript levels for the seven clones were reduced or not detectable in the mutant HG794. Homology search showed that the four cDNA clones, DD-3 an.. Read More
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Seven Tips for Aspiring Biomedical Scientists in a Developing Country
Low-income countries are generally characterized by a disproportionately large share of the global burden of communicable and non-communicable diseases. One would reasonably expect that the solutions to these problems be home-grown. Unaccountably, the capacity for this is wholly lacking, an overwhelming majority of African count.. Read More
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Water Barrier Edible Coatings of Fried Foods
Frying is known as one of the oldest food cooking processes dating back as early as sixteen centuries B.C., being still widespread utilized today at both domestic and industrial scale, because of its ability to enhance flavour and texture of numerous foods. Fried products are appreciated everywhere because of their improved pala.. Read More
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Great emphasis has recently been given to the environmental impacts caused by chemical surfactants due to their toxicity and difficulty in being degraded. Increasing environmental concerns, the advance in biotechnology and the emergence of more stringent laws have led to biosurfactants being a serious alternative to the chemical.. Read More
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At present, titanium-based biomaterials for the production of prosthetic devices have achieved a satisfactory quality level; current research aims at improving their surgical dependability and biomechanical performances. In this contest, a crucial aspect is represented by the osseointegration process, which implies the secure as.. Read More
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Magnetic Resonance Imaging will Replace Computer Tomography
The introduction of tomographic imaging revolutionized the way we look at medical images. Compared to X-ray projective image, Computer Tomography (CT) offers the possibility of analyzing anatomical structures on a three-dimensional, slice-to-slice approach. On the other hand, limitations of CT consist of poor soft tissue contras.. Read More
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A rapid, simple and efficient protocol for in vitro multiple shoot induction and plantlet regeneration was achieved from two different explants of Cicer arietinum cv (ICCCR) (kranthi). The explants viz shoot tip and cotyledonary node were cultured on MS medium fortified with Benzyl Adeno Purine (BAP) (0.5-3.0 mg/L) and Kinetin (.. Read More
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Orchid Micropropagation: Regeneration Competence of Anther Culture
The possibility of using anthers for initiating in vitro cultures of Rhynchostylis retusa (Orchidaceae), has been positively tested. The study was designed to study the effects of physiological status, stage of microspore development and pre-cold treatment on androgenic response in R.retusa. The anthers from open flowers (2 days.. Read More
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Effects of Surface Roughness of Hydroxyapatite on Cell Attachment and Proliferation
Hydroxyapatite (HA) is the main inorganic component of human hard tissue. It is bioactive and supports bone in growth and osteointegration, and thus it is widely used for orthopedic, dental and maxillofacial applications. To fully utilize its potential in forming a strong bone-implant interface, we have investigated the effect o.. Read More
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Intercellular interactions are pivotal for basic cellular activities and errors in either receiving or transmitting these signals are shown to cause pathological conditions. Whilst, such intercellular communications were once thought to be regulated by membrane surface molecules and/or soluble secreted proteins by stimulating th.. Read More
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