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IBrain functions can be affected by the dietary precursors of neurotransmitters. Tryptophan (TRP) is the precursor of serotonin (5- Hydroxytryptamine; 5-HT) neurotransmitter that plays an important role in sleeping, mood, eating behavior, consciousness and cognitive functions. Present study describes the estimation of trypt.. Read More
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Elemental Composition of Drulia browni Collected in Negro River (Amazonas, Brazil)
Sponges, comprising not only organic chemical composition, but also the metals that they filter and accumulate, are aquatic animals that constitute one of the main hotspots in the search for new drugs, being the subject of many studies. The inorganic accumulation pattern from these sponges could be used for several purposes, fro.. Read More
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In this paper a simple, precise and rapid high performance liquid chromatography method with UV detection has been developed for the determination of saxagliptin and metformin in bulk. An Agilent, Zorbax CN (250 × 4.6 mm I.D., 5 μm) column was used with a mobile phase mixture of methanol-50 mM phosphate buffer (pH 2.7) .. Read More
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The analysis of volatile organic compounds (VOCs) emitted from biological fluids such as blood, urine, breath and faeces, has been receiving more attention from researchers and clinicians due to their contribution to the metabolome and potential use as diagnostic tools in clinical settings. The faecal metabolome represents the f.. Read More
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In this work, the potential of activated carbon stems and leaves (ACS, ACL) prepared from dried water hyacinth stems and leaves (DS, DL) by chemical activation with phosphoric acid (1:3) and modified activated carbon stems and leaves (MACS, MACL) with nitric acid (1:1) for the removal of lead from aqueous solution was investigat.. Read More
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The International Conference on Harmonisation [Q2 (R1)] guidelines was applied for this one-step spectrophotometric estimation of chlorpromazine hydrochloride in neurazine tablet formulation. The method was found to be simple, safe, sensitive, and validated for the assay of chlorpromazine hydrochloride using bromophenol blue, ci.. Read More
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Generation of early information on metabolic pathways, metabolite structures and their systemic exposure is a highly time consuming activity during the drug development process. Since these data have become of higher interest for the health authorities, efforts have been made to provide results as early as possible. ViewLux Ultr.. Read More
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Validated spectrophotometric and chromatographic methods have been developed for determination of the antidepressant drug sulpiride (SUL) in pharmaceutical formulation and plasms. The new spectrophotometric methods were based on the formation of sulpiride yellow ion-pair complex with bromocresol green (BCG), congo red (CR) or me.. Read More
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Cancer is a group of diseases characterized by uncontrolled growth and spread of abnormal cells, which cause high mortality rates. The purpose of this study is to characterize the proteins secreted from the HepG2, MCF-7 and HT-29 cells, which may relate to cell differentiation and tumor metastasis. In the proteomic analysis, the.. Read More
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Comparison of Sampling Probe and Thermal Desorber in HAPSITE ER for Analysis of TO-15 Compounds
The Hazardous Air Pollutants on Site (HAPSITE), a portable Gas Chromatograph-Mass Spectrometer (GCMS), has been used to detect, identify, and quantify Volatile Organic Compounds (VOCs) from environmental samples, providing on-site analysis to aid in operational risk management. HAPSITE is equipped with a hand-held sampling probe.. Read More
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Glyphosate is a broad-spectrum herbicide widely used for eliminating weeds in crop fields. Its mode of action is believed to be via translocation from the source to the sink tissues where it then interferes with the activities of 5-enol-pyruvylshikimate-3-phosphate synthase (EPSPS). In this study, the translocation of glyphosate.. Read More
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Assay for Glycosaminoglycans by Tandem Mass Spectrometry and its Applications
Glycosaminoglycans (GAGs) are distributed in the whole body and play a variety of important physiological roles associated with inflammation, growth, coagulation, fibrinolysis, lipolysis, and cell-matrix biology. Accumulation of undegraded GAGs in lysosomes gives rise to a distinct clinical syndrome, mucopolysaccharidoses. Measu.. Read More
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The presented data are of practical importance in terms of vitamin E determination in meat and other foodstuffs. Selection of a proper separation and detection method as well as sample preparation protocol might affect the reliability of results. Application of a sensitive method and such sample preparation procedures that do no.. Read More
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A high performance liquid chromatography method for the determination of fenofibric acid (FA), the active form of fenofibrate (FBT) in human plasma was developed and validated with 500 μL of human plasma using 4’-chloro- 5-fluro-2-hydroxybenzophenone (CFHB) as internal standard (IS). The assay procedure involved a simpl.. Read More
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Development of an On-line GC System using Existing Retired Equipment
To ensure drinking water quality, continual monitoring of the volatile organic compounds (VOCs) in the source water and the treated water is very important for any water treatment plant. On-line, real-time information is crucial to water treatment engineers and operators. The concentration of VOCs provides such information. For .. Read More
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Optical and Electrical Si-Based Biosensors: Fabrication and Trasduction Issues
In this work we discussed feasibility studies and examples of biological molecules integration in Si-based miniaturized devices. We investigated three main issues: (i) device surface functionalization, (ii) biological molecule functionality after immobilization and (iii) biosensor working principle using both electrical and opti.. Read More
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A rapid, simple and sensitive liquid chromatography/tandem mass spectrometry (LC-MS/MS) assay was developed for the determination of total and free concentrations of flucloxacillin and cefazolin in human plasma. Free flucloxacillin and cefazolin were separated from bound drug by ultra filtration. Samples of plasma and plasma ult.. Read More
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Analysis of Pesticides in Water Samples and Removal of Monocrotophos by γ-Irradiation
The analysis of 20 organochlorine pesticides has been carried out in surface water samples collected from Peshawar, KPK, Pakistan, using SPME-GC-ECD method. This method showed good liner response with R2 values in the range of 0.9933 to 0.9999 for all pesticides. Percent recoveries at 1 μg L-1 for all the pesticides ranged fr.. Read More
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This study is intended to determine if experimental design and Monte Carlo simulation methods can be utilized to optimize the liquid chromatography (LC) analysis of active molecules. The method was applied for the simultaneous analysis of two vaginal microbicides, stampidine (STP) and HI443 in bulk and nanoformulations. The Plac.. Read More
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In the present work, bentonite (Bn) clay, from Egypt, has been modified using HCl to produce the acid-activated bentonite (A-ABn). Both Bn and A-ABn adsorbents have been characterized using Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and nitrogen adsorption measurements. The prepared adsorb.. Read More
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