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For low protein concentrations containing biological samples (in proteomics) and for non proteinaceous compound assays (in bioanalysis), there is a critical need for a simple, fast, and cost-effective protein enrichment or precipitation method. However, 2,2,2-trichloroacetic acid (TCA) is traditionally used for protein precipita.. Read More
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Mass Spectrometry: A Boon to Nuclear Industry
Mass spectrometry is a versatile technique that is used for the determination of elements at the trace and ultratrace level along with the isotope ratios. The accuracy in the quantification of isotope ratios of radionuclides is essential for environmental monitoring, migration studies, dating, determination of burn-up of fuel, n.. Read More
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A recent finding showed that ceramide and sphingosine-1-phosphate (S1P) become exposed on the surface of cells treated by photodynamic therapy (PDT) and acquire the capacity to act as danger-associated molecular patterns (DAMPs). To explore this further, the present study examined whether ceramide and S1P can be released from PD.. Read More
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Cashew crop grown in the semi-arid conditions of tropical countries produces cashew nut, an important commodity both for internal consumption and exportation. Cashew is very well adapted to abiotic stresses such as drought, high temperature, high salinity, and solar radiation predominant in the environmental regions where cashew.. Read More
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A rapid and sensitive liquid chromatography–tandem mass spectrometry (LC–MS/MS) method in human plasma was developed for determination of levonorgestrel using ultra performance liquid chromatography aligned with tandem mass spectrometric detection. The analytes were extracted using solid–phase extraction techni.. Read More
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Harmonized collaborative validation of a simultaneous and multiple determination method for nivalenol, deoxynivalenol, T-2 toxin, HT-2 toxin, and zearalenone in wheat and barley by liquid chromatography tandem mass spectrometry (LC-MS/MS) was conducted by participants from 12 laboratories. The fortified samples of wheat and barl.. Read More
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Many photosensitizers, including chlorins, are highly hydrophobic, which makes intravenous administration problematic and affects their delivery to the tumor and uptake in the cells. Moreover, self-aggregation of the photosensitizer in aqueous solution reduces fluorescence quantum yield, triplet state, and singlet oxygen generat.. Read More
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Fundamentals and Applications of Impedimetric and Redox Capacitive Biosensors
Electroanalyses have brought a huge amount to our understanding of interfaces generally. When applied to surfaces which have been specifically engineered so as to recruit targets from an analytical solution, potent sensors can be derived. These may be based on a multitude of different analytical methods all typified by specific .. Read More
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A Novel Approach for Microsensing: Detecting and Identifying Eigenmodes of Sensing Objects
A novel and straight forward approach for analysis of whispering gallery-mode micro cavity sensing is presented using the finite difference time-domain (FDTD) method. The FDTD simulation shows that eigenmodes of sensing objects (SOs) at the micro-scale can be detected as SO signatures, and therefore provide more accurate and rob.. Read More
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Membrane Bound Molecular Machines for Sensing
This paper reports on the construction and predictive models of the Ion Channel Switch (ICS) biosensor which is capable of detecting femto-molar concentrations of target species including proteins, hormones, polypeptides, microorganisms, oligonucleotides, DNA segments, and polymers in cluttered electrolyte environments. The ICS .. Read More
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Acetone in exhaled breath is a potential biomarker of diabetes mellitus (DM) and elevated breath acetone concentrations have been observed in DM patients. One of the near real-time and online breath acetone analysis techniques is cavity ringdown spectroscopy (CRDS) that has been demonstrated for breath acetone measurements in hu.. Read More
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We report herein, 3-aminopropyltrimethoxysilane (3-APTMS) and 3-glycidoxypropyltrimethoxysilane (3-GPTMS) mediated reduction of graphene oxide into graphene. The resulting graphene has been characterized by UV-VIS and Raman spectroscopy. The as synthesized graphene is used to make polycrystalline nanocomposite of prussian blue h.. Read More
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Physico-Chemical Stability of Mixtures of Fosaprepitant used in Clinical Practice
Fosaprepitant dimeglumine is a new drug indicated to prevent nausea and vomiting associated with highly emetogenic cisplatin-based and moderately emetogenic cancer chemotherapy in adults. Due to its complexity in managing, since it requires reconstitution and dilution before intraveonous administration, it is necessary to evalua.. Read More
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A liquid chromatography/tandem mass spectrometry (LC-MS/MS) assay was developed for the determination of vancomycin in human plasma, bone and fat tissue. For vancomycin in plasma, sample was treated with methanol to precipitate the proteins. After centrifugation, the supernatant was diluted with water, and then injected into the.. Read More
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Propofol is an important compound used for anaesthetic purposes in clinical practice. Nevertheless, in the recent years, the use of propofol has also been reported for recreational, abusive or even for suicidal and criminal purposes. So far, there is a lack of practical techniques validated for simultaneous quantification o.. Read More
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Mass spectrometry (MS) offers an exciting possibility as a highly specific method for measuring vitellogenin (VTG) concentrations in toxicity tests that are aimed at evaluating perturbations in the endocrine system. Typically MS approaches to protein quantification attempt to measure intact proteins or rely on enzymatic digestio.. Read More
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Fast and selective liquid chromatography-mass spectrometry (LC-MS) method was developed for the quantification of posaconazole in different mouse organs including liver, heart, brain, kidney and lung. Organs were homogenized and diluted in isotonic NaCl solution. Protein was precipitated using acetonitrile containing internal st.. Read More
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The theme of this study was the preparation, characterization, and application of a polyaniline (PAN) and graphene composite modified glassy carbon electrode (PAN/Gr/GCE) for the voltammetric determination of doripenem (DPM) and meropenem metabolites (MPM) in human urine and serum samples. The morphological study of the PAN.. Read More
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ICP-AES Ca and Sr ion line signals were measured on 0-30 μg/mL Ca and Sr solutions, in the absence and presence of 1000 μg/mL Li as easily ionizable interferent, and the effect of the interferent on analyte calibration curve simulated using a simplified collisional radiative recombination rate model. Close agreement betwee.. Read More
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Efficient Removal of Phenol from Water Samples Using Sugarcane Bagasse Based Activated Carbon
Sugarcane bagasse-based activated carbons (SCBACs) with different chemical characteristics appropriate for the removal of phenol in aqueous solutions have been prepared. The steam activated carbon (SCBACS) is obtained from the carbonized sugarcane bagasse residues in the presence of nitrogen in the temperature range from 70.. Read More
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