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Sodium dodecyl sulphate/poly(brilliant blue)/multi walled carbon nanotube modified carbon paste electrode was fabricated for the electroanalysis of dopamine in the presence of ascorbic acid and uric acid in phosphate buffer solution of pH 7.4. The key parameters such as sensitivity, selectivity, antifouling property and stabilit.. Read More
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A novel HPLC method has been developed and validated for the determination of Latanoprost in pharmaceutical ophthalmic formulation for in-vivo and ex-vivo animal studies. The method used a Supelco analytical C18 (250 mm × 4.6 mm i.d., 5 μm particle size) column, under the following chromatography conditions: Isocra.. Read More
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Although acetone, in DESI ionisation generally leads to protein aggregation, in this study we report unexpected multi-proton transfers to lysozyme using this aprotic solvent as a charged spray. The DESI/acetone mass spectrum of lysozyme displays (i) a significant increase in the average charge state (Zav) and (ii) an incomplete .. Read More
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Determination of Sulfur in Bio-Samples by ICP-QMS/QMS with an ORC
A method for determination of sulfur was investigated using an inductively coupled plasma tandem quadrupole mass spectrometer (ICP-QMS/QMS) with an octopole reaction/collision cell (ORC). It helped to achieve a lower background equivalent concentration (BEC) and a lower detection limit (DL) when 10% HNO3 with 10% ethanol was app.. Read More
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Quantification of Sugar Epimers in Polygalactomannans by ESI-MS/MS
Polygalactomannans (PGMs) represent an important family of polysaccharides. They are obtained from the endosperm of leguminous plant seeds and are employed in a growing number of industrial applications as thickening agents and rheology modifiers. Each PGM has a typical mannose/galactose (M/G) ratio (from ~1 to ~5), which determ.. Read More
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Application of X-ray Fluorescence Spectrometers for Biopharmaceutical Forensics
In biopharmaceutical drug development and production, a portable energy dispersive x-ray fluorescence (EDXRF) spectrometer can be used for identification of foreign particles, investigation of discolored solutions, and improvement of process understanding. The spectrometer is a safe, small, robust, rapid, easy-to-use, multieleme.. Read More
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The separation of the two components pulp-stone has allowed the development of a more scented product that respects the environment. According to a preliminary study, it was noticed that the destoning process produced a paste containing 67% moisture without resorting to the addition of water. The destoning process presents an ec.. Read More
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The taxonomic identification of mushrooms suspected to contain hallucinogenic active principles was carried out using a DNA-based approach, thus highlighting the usefulness of this approach in the forensic identification of illegal samples also when they are difficult to identify because the morphologic identification is prevent.. Read More
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Raman Microspectroscopy Demonstrates Alterations in Human Mandibular Bone after Radiotherapy
Quality and alterations in the biochemical composition of bones used for dental implantation after radiotherapy in cancer patients is always a critical and debatable factor. Clinically the irradiated bone is similar to control bone. The aim of this study was to verify any compositional alterations in human mandible bone after ir.. Read More
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Nutraceuticals from Olives Plain Water Extraction, Identification and Assay by LC-ESI-MS/MS
Ancient olive trees are widespread in many countries of the Mediterranean area, including the Calabria region located in southern Italy. They have adapted to environments, climates and soil conditions and represent an ecosystem heritage. The use of their drupes for manufacturing both table olive and oil is not often economically.. Read More
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Nicotine as Corrosion Inhibitor for 1018 Steel in 1M HCl under Turbulent Conditions
An electrochemical impedance technique for determining corrosion inhibition of nicotine in HCl on AISI 1018 steel under concentrations from 0 to 50 ppm found that the organic compound is a better corrosion inhibitor under static conditions. The inhibition efficiency (IE) increased with inhibitor concentration reaching an IE>9.. Read More
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Sodium alpha olefin sulphonate (SAOS) was used for the modification of carbon paste electrode (CPE) to determine the electrochemical behavior of folic acid (FA) in 0.2M phosphate buffer solution (PBS) at pH 7.4 with the scan rate of 50 mVs-1. The effects of scan rate, concentration and simultaneous determination of FA at modifie.. Read More
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Saccharides such as glucose, galactose and amino acids like lysine, phenylalanine are known as bio-markers of metabolic disorders, and several analytical methods have been developed to determine these bio-markers in blood and urine by enzymatic method, HPLC analysis and so on. Multi-biosensing systems for simultaneous determinat.. Read More
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Diagnostic Specificity of Nickel Nano-Particles in Allergic Contact Dermatitis
Metal allergy is mainly an environmental disorder. Nickel is present everywhere. Exposure to nickel is a major cause of Allergic Contact Dermatitis, which respectively is an inflammatory, cellular type delayed hypersensitivity immuno response induced by antigen specific T cells. However, there is no standardized method to assess.. Read More
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Effect of Somatic Cell Count on Bovine Milk Protein Fractions
The objective of this study was to evaluate the influence of somatic cell count (SCC) on the physicochemical properties and protein fractions of milk. Milk was collected and analyzed for somatic cell count, fat, lactose, acidity, total solids, ash, total nitrogen, soluble nitrogen at pH 4.6, and soluble nitrogen in trichloroacet.. Read More
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Diversity of biological samples is still partially considered by conventional Differential Interference Contrast (DIC) microscopy approaches. Here we propose a new algorithm (developed as an ImageJ macro), the STA (Shadow Technique Algorithm), whose originality relies in the 3D/4D visualization of a large range of biological obj.. Read More
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A Novel [15N] Glutamine Flux using LC-MS/MS-SRM for Determination of Nucleosides and Nucleobases
The growth of cancer cells relies more on increased proliferation and autonomy compared to non-malignant cells. The rate of de novo nucleotide biosynthesis correlates with cell proliferation rates. In part, glutamine is needed to sustain high rates of cellular proliferation as a key nitrogen donor in purine and pyrimidine nucleo.. Read More
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BMCL26 is a potential drug derived from nimesulide, which has exhibited the substantial anti-parasitic activity in various cell lines. To conduct various pharmacological and toxicological properties of this drug, we developed and validated a rapid LC-MS/MS method for its quantification in accordance with the FDA guidelines. Prot.. Read More
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Spectroscopic Characterization of Disulfiram and Nicotinic Acid after Biofield Treatment
Disulfiram is being used clinically as an aid in chronic alcoholism, while nicotinic acid is one of a B-complex vitamin that has cholesterol lowering activity. The aim of present study was to investigate the impact of biofield treatment on spectral properties of disulfiram and nicotinic acid. The study was performed in two group.. Read More
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Being the fourth most important crop in the developing countries surpassed only by maize, rice and sugarcane as a source of calories, cassava (Manihot esculenta Crantz) is doubtlessly a famine reserve crop due to its drought tolerance, ability to grow on infertile soils and its ability to recover from disease and pest attacks. H.. Read More
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