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Non-Aqueous Titrimetric Assay for Determination of Loratadine in Pharmaceutical Preparations
Non-aqueous titrations is the most titrimetric procedure used in pharmacopoeial assays and serves a double purpose, as it suitable for the titration of weak acids and bases and provides a solvent in which organic compounds are soluble. A simple, cost-effective, rapid, and precise method for determination of loratadine (LOR) in p.. Read More
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This paper presents the improvement of analytical methods for intramolecular carbon isotope compositions (δ13C) of pyruvate. Decarboxylated by H2O2, pyruvate yields acetic acid and CO2. Headspace solid phase micro-extractiongas chromatography-pyrolysis-gas chromatography-combustion-isotope ratio mass spectrometry (HS-SPME-.. Read More
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Total Reflection X-Ray Fluorescence Spectroscopy to Evaluate Heavy Metals Accumulation in Legumes
This work is to demonstrate the usefulness of total reflection X-ray fluorescence (TXRF) for fast and reliable quantitative analysis of heavy metals in plants used for accumulation studies. A model study of beans germination in lead contaminated environment under controlled laboratory conditions was realized. Metal accumulation .. Read More
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TLC densitometry method for quantification of gallic acid and ellagic acid using HPTLC is developed. This is the first report of quantification of these two biologically active compounds as gallic acid and ellagic acid using HPTLC from this plant. Quantification of gallic acid and ellagic acid was carried out from the methanol e.. Read More
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A liquid chromatography mass-spectrometric method is described for the simultaneous estimation of 9-cisretinoic acid and 4-oxo-9-cis-retinoic acid in human plasma using 13-cis-retinoic acid-d5 as an internal standard. Chromatographic separation of these retinoids was achieved on Chromolith Performance RP18 (100 × 4.6 mm) c.. Read More
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To evaluate CoQ10 concentration a UPLC/MS-MS method for its analysis in plasma samples was developed. Before validation, the choice of the anticoagulant, of the Internal Standard (CoQ9; IS) and the stability of CoQ10 in plasma were evaluated. These determinations were necessary to assess the effect of the oral intake of CoQ10 in.. Read More
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Label-free detection using arrays of SiO2 nanospheres (of diameters ~500 nm) core and gold nanoparticle seeding (~4 nm) shell, with additional gold electroplated films were used as biosensors for adsorption of human fibrinogen. Also, surface enhanced Raman spectroscopy (SERS) of fibrinogen was studied as a function of the thickn.. Read More
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A commercially-available mono-sialo (GM1) ganglioside standard consists of three major components with different ceramide structures: C18:0 fatty acid/C18-sphingosine, C18:0 fatty acid/C20-sphingosine and C20:0 fatty acid/C18-sphingosine. The usual multiple reaction monitoring (MRM) liquid chromatography-tandem mass spectrometry.. Read More
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A rapid, precise and selective RP-LC method was developed for simultaneous determination of the widely used oral antidiabetic; metformin hydrochloride (MTF), with some commonly prescribed oral antidiabetics, namely; sitagliptin phosphate (SIT), pioglitazone hydrochloride (PGZ), gliclazide (GLZ), glibenclamide (GLB) and repaglini.. Read More
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One of the most challenging aspects of interpreting quantitative information of biological samples from laser inductively coupled plasma mass spectrometry (LA-ICP-MS) is a lack of appropriate matrix-matched internal standards that is needed for calibrations. There are standards available; however, most certified reference standa.. Read More
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A Rapid ELISA Method to Improve the Automated Test Throughput
In ELISA, ligand is commonly adsorbed to the plastic surface through non-covalent bonds between the hydrophobic regions of the ligand and the plastic surface. Thus, all the reactions occur in a heterogeneous phase, with some reactants in solution, and some immobilized. As a result, the diffusion constant of immobilized reagents .. Read More
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A fundamental study of peptide transport behavior provides information about length dependent transport and sequence structure relationships. In these highly accurate investigations of peptide electrokinetic properties and peptide conformations using a Micro-Electro-Mechanical Systems (MEMS)-fabricated capillary zone electrophor.. Read More
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A device that can detect the concentration of total organic carbon (TOC) continuously was designed by combining the technology of supercritical water oxidation (SCWO) and non-dispersive infrared analyzer (NDIR). CuSO4, as one of the common homogeneous catalysts in the SCWO of wastewater, was introduced into the device to lower t.. Read More
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Matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been proposed as a technique of choice for quick classification of bacterial cells and precise characterization of microorganisms. The evaluated MALDI MS procedure was confronted with automated biochemical method using the VITEK 2 Com.. Read More
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The activated carbon samples (SC and PC) were prepared from papyrus plant by chemical activation with sulfuric acid and phosphoric acid, respectively. The SC and PC were then modified with nitric acid to give the oxidized sulfuric carbon (OSC) and oxidized phosphoric carbon (OPC) and utilized for removing Methylene Blue (MB) fro.. Read More
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A Novel Catechol Electrochemical Sensor Based on Cobalt Hexacyanoferrate/ (CoHCF)/Au/SBA-15
A novel electrochemical sensor for catechol was developed by electrodepositing HAuCl4 and cobalt hexacyanoferrate (CoHCF) on an ordered mesoporous SBA-15 decorated glassy carbon electrode (GCE). The CoHCF/Au/SBA-15 film was characterized by scanning electron microscopy (SEM) and impedance spectra. A mesoporous SBA-15 was used as.. Read More
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Rapid Analysis of Cocaine in Saliva by Surface-Enhanced Raman Spectroscopy
Increases in illicit drug use and the number of emergency-room visits attributable to drug misuse or abuse highlight the need for an efficient, reliable method to detect drugs in patients in order to provide rapid and appropriate care. A surface-enhanced Raman spectroscopy (SERS)-based method was successfully developed to rapidl.. Read More
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This study plans to functionalize activated carbon with oxygen and nitrogen containing groups to improve removal of Cd(II) ions from aqueous solutions. Activated carbon (AC) was treated with nitric acid giving MC-HNO3 that was pursued by modifying with EDTA to form MC-EDTA. AC, MC-HNO3 and MC-EDTA were analyzed using FTIR, .. Read More
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Streptokinase (STK) is a thrombolytic agent clinically used to treat patients with acute myocardial infarction and venous and arterial thrombosis. An in vitro chromogenic substrate end point bioassay was validated for the potency evaluation of biopharmaceutical formulations. The dose-response curve was linear over the concentrat.. Read More
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Surface-enhanced Raman scattering (SERS) has long since found wide application in the field of heterogeneous catalysis for both chemical and photochemical reactions. Actually, this technique allows the in-situ detection of reactants or products or by-products in reactions that take place at the surface of nanostructured metals l.. Read More
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