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A simple and sensitive HPLC method was developed and validated for the study of in-vitro drug release from haloperidol loaded solid lipid nanoparticles (SLNs). The method was also used for the analysis of drug for detection of shelf life of developed SLNs. Chromatogram separation was achieved using C18 column as stationary p.. Read More
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Dynamic Light Scattering and Taylor Dispersion Analysis are two methods employed for the measurement of the diffusion coefficients and interaction parameters of solutes. For self-associating solutes, the interaction parameter provides a measure for the degree of association and hence the stability of the solutes. Due to th.. Read More
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In this study, we have developed a novel and efficient method based on spectrophotometry in combination with first-order multivariate calibration for simultaneous quantification of cholesterol (CHL) and cholestanol (CHN) in human serum samples. Several multivariate calibration (MVC) models including partial least squares-1 (.. Read More
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Many over-the-counter glucose measurement systems currently exist but are not widely used by nondiabetic consumers because of the inconvenience. There exists a need for new methods of conveniently detecting early stages of diabetic or prediabetic conditions rather than waiting for the disease to progress to the point that symp.. Read More
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Mycotoxicological Concerns with Sorghum, Millet and Sesame in Northern Nigeria
Incidence of fungi and aflatoxin in sorghum, millet, sesame and their products in Northern Nigeria was investigated in 146 food samples including; sorghum and traditional beer (50), millet and millet dough (50), and sesame seed (50). Members of the Aspergillus, Fusarium, Pennicilium, Macrophomena, Cercospora, Phoma, Rhizop.. Read More
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Hibiscus sabdariffa tea is a widely used medicinal beverage and a treatment for high blood pressure and high blood cholesterol in many parts of the world. Many studies on H. sabdariffa have been conducted including extraction and identification of main biocompounds. However, information on the effects of processing the plant.. Read More
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In this work, a method of single drop microextraction (SDME) combined with high performance liquid chromatography (HPLC) with diode array detection (DAD) was studied for trace level enrichment as well as simultaneous determination of atrazine (ATZ) and its major degradation products such as desethylatrazine (DEA) and desisopr.. Read More
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Quantitative Determination of Topiramate in Human Breast Milk
A thin-layer chromatographic (HPTLC) method for quantification of topiramate in human breast milk was developed using liquid –liquid extraction with n-hexane and methanol as extraction solvents, fluorescence activation with ninhidrine (1% ethanolic solution) and chlorpromazine as internal standard. Thin-layer chromatograp.. Read More
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Features of N-Glycosylation of Immunoglobulins from Knockout Pig Models
For the first time, the N-glycosylation patterns of immunoglobulin G (IgGs) isolated from the serum of two varieties of knockout pigs (lacking N-glycolylneuraminic acid (Neu5Gc) and/or α 1,3 galactose) were examined for the presence of potential glycan xenoantigens and compared to N-glycosylation patterns obtained for .. Read More
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Bioassay of Lindane (Gamalin 20) to Hetrobrancus bidorsalis Juveniles
The study was carried out to determine the acute toxicity of lindane (Gammalin 20) to Hetrobranchus bidorsalis juveniles using static bioassays. The mean weight and total length of the fish samples were 2.5 g and 8.6 cm respectively. Six groups of the experimental units were set up containing 10 fish individuals in each bowl wit.. Read More
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In the present study, a cloud point extraction method was used for the preconcentration and extraction of cadmium (II) and zinc (II) ions in different environmental samples. The zinc and cadmium ions formed hydrophobic complexes with phenanthraquinone monophenyl thiosemicarbazone (PPT). These complexes were extracted using Trito.. Read More
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This study evaluated the label-free bio-layer interferometric (BLI) biosensor for the detection of norovirus (NoV) using two types of virus like particles (VLPs) that represent human NoV GI.1 and GII.4. To construct biosensors for NoV GI.1 and GII.4 detection, the commercial AMC sensors, on which anti-mouse Fc-specific antibodie.. Read More
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The in-situ local corrosion behavior of scratched epoxy coated carbon steel is investigated in sat. Ca(OH)2 with varying concentration of Cl- ions by localized electrochemical impedance spectroscopy (LEIS). The localized corrosion process and mechanism of coated steel (scratch area) is measured by LEIS plots and 3D topographic.. Read More
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N,N-Dimethylacetamide (DMA) is an excellent solvent that is highly used in the production of synthetic fibres as well as in the pharmaceutical industry. It is present as a solvent in the intravenous formulation of busulphan, a drug used in high doses as myeloablative treatment prior to hematopoietic stem cell transplantation (SC.. Read More
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Removal of Fluoride from Aqueous Solution Using Chitosan-Iron Complex
In this study highly efficient sorbent, chitosan-Fe3+ complex with high chemical stability material was synthesized and the performance towards Fluoride adsorption was evaluated by batch experiments. The adsorption process reached equilibrium at 1 hour. The maximum adsorption capacity reached 2.34 mg/g of F- at an initial conce.. Read More
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Development of Analytical Methods to Gain Insight into the Role of Terpenes in Biogas Plants
Terpenes have been shown to be the dominant VOCs present in biogas mostly in plants where food wastes are digested. In particular, p-cymene and D-limonene have been reported to represent up to 90% of all VOCs in the biogas. A number of problems have been linked to terpenes in biogas plants, including odor problems, indoor air qu.. Read More
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This review covers the principles of two dimensional gas and liquid chromatography (2DGC and 2DLC) and briefly introduces the theory accounting for the increase in separation resulting from a greater peak capacity than for the one dimensional (1D) mode. The advance in the techniques from multi-dimensional to comprehensive chroma.. Read More
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A new highly selective optical sensor was prepared by de-esterification of triacetyl cellulose transparent film and chemical immobilization of 1-acenaphthoquinone 1-thiosemicarbazone (L) on it. The absorbance variation of immobilized 1-acenaphthoquinone 1-thiosemicarbazone on hydrolyzed cellulose acetate film of upon addition of.. Read More
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The growth of single, self- nucleated calcium carbonate crystals on a polysulfone (PSU) film was investigated with high resolution, time lapse Raman imaging. The Raman images were acquired on the interface of the polymer with the crystal. The growth of crystals could thus be followed in time. PSU is a polymer that is used as a m.. Read More
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Bleaching Edible Oils Using Clay from Kangole, Moroto District, North Eastern Uganda
The search for natural clays that can be used to provide industrial bleaching earths is worldwide; Uganda’s rich volcanic clays from North Eastern region remain unutilized. The aim of the study was to evaluate the use and efficiency of clay mined locally at Kangole to bleach vegetable oils when activated by leaching in hyd.. Read More
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