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tFRAP: A FRAP-Based Technique to Monitor Protein Translation in Living Cells
Traditionally, studies on protein translation rely on systems, in which cells have been lysed prior determination of levels of the protein of interest. However, these assays do not reflect the protein synthesis in living cells in real time, but analyze protein levels after a given incubation time, leading to limitations in resul.. Read More
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Formation of Polyelectrolyte Complexes from Cationic Polyfluorenes and ssDNA
Trimethylphosphonium-substituted polyfluorenes (PTMPHFs) with controlled molecular weights were synthesized using the Kumada catalyst-transfer polycondensation procedure, and their recognition properties towards various biomolecules were examined. Upon the formation of polyelectrolyte complexes with single stranded DNA (particul.. Read More
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The purpose of this study was to evaluate a potential pharmacokinetic interaction between antipsychotics (quetiapine QUE, clozapine CLO and olanzapine OLA) and beta-blockers (metoprolol MET, propranolol PRO, bisoprolol BIS, nebivolol NEB and carvedilol CAR). Antipsychotics and beta-blockers are metabolized by the same cytochrome.. Read More
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Although breath acetone (BA) has been identified as a breath biomarker for some abnormal metabolic status, such as diabetic ketoacidosis, diabetes under insulin treatments, on a ketogenic diet, heart congestion failure, and post intense exercises, many intra-individual biological parameters also influence the breath acetone conc.. Read More
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Removal of Methylene Violet from Aqueous Solutions Using BaSr2NbO5.5
The removals of Methyl violet from aqueous solutions using the double perovskites BaSr2NbO5.5 are reported. The oxides were prepared using solid state reaction in which the raw materials were mixed initially by either acetone or water. The X-ray diffraction measurements demonstrated that the preparation methods have influenced t.. Read More
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Seventeen Organochlorine Pesticides (OCPs) were evaluated in 14 surface sediment samples from a dam lake in Northwestern Turkey. As analytical tool GC-Mass system; HP (Hewlett Packard) 6890 series gas chromatograph coupled with HP 5973 mass spectrometer was used. The HP 5MS capillary column had 30 m length with 0.32 mm internal .. Read More
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A SnO2 electrode material was successfully synthesized using the Pechini method and is of interest for potential use in lithium batteries. In this work, the effects of the calcination temperature on the fabrication of the SnO2 electrode material were investigated in detail. The architecture, morphology and crystal phase of the S.. Read More
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Stability and Concentrations of Humectants in Tobacco
Humectants, especially glycerol and propylene glycol, are tobacco additives that are used to facilitate several processes in the production of tobacco products and to maintain the moisture content. Humectants in tobacco are usually detected by gas chromatography with flame ionization detection after a pre-analytical conditioning.. Read More
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To improve water quality, there should be a mechanism of keeping safe water source from chemical contaminants in an effective and protective way through the application of regular check up and with interventions by taking exact measure periodically before it is supplied for usage. The intention of this research work is to determ.. Read More
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For the development of a histamine (HIS) potentiometric sensor based on molecularly imprinted polymers (MIPs), the effects of four functional monomers, namely acrylamide (AA), atropic acid (AT), methacrylic acid (MAA), and 4-vinylpyridine (4-VP), from which the MIP was synthesized, on the performance of the HIS sensor were exami.. Read More
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Seasonal and spatial variation and sources of phase-distributed polycyclic aromatic hydrocarbons (PAHs) in Northwestern part of Turkey was studied for pollution level and sources appointment. Daily sampling of air was done for one year in two sites; Balıkesir city and the dam lake. Samples were analyzed by using GC-MS. T.. Read More
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Schisandra chinensis (SC) is a well-known traditional Chinese herbal medicine that has been used in clinical practices for thousands of years. However, the differences between the effects of unprocessed and vinegarprocessed Schisandra chinensis (VSC) on cytochrome P450 (CYP450) activities are poorly understood. To evaluate t.. Read More
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The knowledge of environmental hazards resulted from natural radioactivity and human activities is very important for monitoring of environmental contamination. In this study, the environmental hazards due to the natural radioactivity and human activities in Tuban delta in Yemen were discussed and evaluated using gamma ray s.. Read More
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This study was conducted to purification G6PD enzyme from diabetic patients by using simple and cheap method the technique gel filtration on Sephadex G100 and determine molecular weight of enzyme and compare it with true molecular weight of enzyme and determine kinetic constant (Km, Vmax) and study the effect of temperature .. Read More
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Background: Adenosine is a nucleoside that impacts the cardiovascular system during cardiovascular or inflammatory diseases. The rapid determination of adenosine in blood may be useful in emergency medicine especially in syncope diagnose or septic shock. We compare its measurement in blood using fixed potential amperometry (FPA).. Read More
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Rapid Identification of Buprenorphine in Patient Saliva
Buprenorphine is becoming the medication of choice to help patients withdraw from opioid addiction. However, treatment is compromised by the inability of physicians to assess patient usage during scheduled examinations. Here we describe the development of a point-of-care (POC) analyzer that can rapidly measure both illicit a.. Read More
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The determination of the seven elements was performed by Perkin Elmer Atomic Absorption (AA) spectrophotometer. The present study highlights the importance of seven heavy metals residual concentration including Cd, Cr, Cu, Fe, Mn, Ni and Zn in milk of Camel, Cattle, Buffalo, Sheep and Goat from various areas of Khyber Pakhtu.. Read More
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Definition of System Suitability Test Limits on the Basis of Robustness Test Results
A chromatographic method newly optimized to identify and assay four antihypertensive drugs in tablet dosage forms was complemented by a robustness test. The best system suitability criteria for numerous responses were evaluated on the basis of the robustness test results. Generally speaking, it is difficult to achieve a total sa.. Read More
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Selected Reaction Monitoring: A Valid Tool for Targeted Quantitation of Protein Biomarker Discovery
Biomarker discovery is relying on the sensitivity and specificity of the detected biomolecules in clinical samples. Several potential biomarkers against the particular disease cannot be detected because of the unavailability of either specific procedure or sensitive instrument or both, which hamper the diagnosis. In spite of.. Read More
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Analysis and Preparation of Linaclotide by High Performance Liquid Chromatography
Linaclotide, a synthetic peptide, is a guanylate cyclase-C (GC-C) agonist for treating irritable bowel syndrome with constipation and chronic idiopathic constipation. A simple, precise and selective RP-HPLC method for linaclotide analysis was developed and validated. Analysis was achieved with a SinoChrom ODS-BP column, mobile.. Read More
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