Computational Analysis of Mutations in Colon Cancer Genes Reveals a Possible Role of Micro satellite in Mutagenesis |
Allam Appa Rao1, G R Sridhar2, Suresh B Mudunuri1, E Vamsidhar1, Gunna Kishore1* |
| 1Department of Computer Sciences and System Engineering, Andhra University,
Visakhapatnam, India |
| 2Endocrine and Diabetes Centre, Visakhapatnam |
| *Corresponding author: |
Dr. Gunna Kishore
E-mail: kishore_brbm@yahoo.co.in |
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| Received April 20, 2008; Accepted May 15, 2008; Published May 25, 2008 |
| Citation: Allam AR, Sridhar G R, Suresh BM, Vamsidhar E, Gunna K (2008) Computational Analysis of Mutations in Colon Cancer Genes Reveals a Possible Role of Micro Satellite in Mutagenesis. J Proteomics Bioinform S1: S041-S045. doi:10.4172/jpb.s1000007 |
| Copyright: ©2008 Allam AR, etal. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| Abstract |
Computer science is a subject, which deals with the manipulation of data so that new data, implicit in the original, appear in a
useful form. We have used the analogy of genome analysis and VIRUS (vital information recourse under siege) and analyzed
MLH1, MSH2 and MSH6 gene which play an important role in repairing mistakes made in DNA replication in colon cancer. If
the MLH1, MSH2, MSH6 proteins are mutated and therefore don’t work properly, the replication mistakes are not repaired,
leading to damaged DNA. The information of all the experimentally proven mutations were collected and analyzed using
bioinformatics tools and software programs. We tried to find out whether the presence of or simple sequence repeats in the
MLH1, MSH2, MSH6 gene has any significance in the generation of these mutations and checked whether these mutations are
fallen in the regions of those microsatellites and if so is there any significance of these microsatellites in the functional domains of
the each gene. Our analysis reveled that 3 of the 10 mutations of the MLH1 gene and all the 10 mutations of the MSH2 gene and
the one mutation in the MSH6 gene that are existing in the microsatellite regions are fall.en in the domain regions of the respective
genes and thus indicating a positive role of microsatellites in mutagenesis. |
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