Prediction of 3-Dimensional Structure of Cathepsin L Protein
of Rattus Norvegicus |
| Sunil Kumar*, Priya Ranjan Debata, Prakash C. Supakar* |
| Institute of Life Sciences, Nalco Square, Bhubaneswar-751023, India |
| *Corresponding authors: |
Sunil Kumar, M.Sc., Institute of Life Sciences, Nalco Square,
Bhubaneswar-751023, India;
Phone: 91-674-2301500,
Fax: 91-674-2300728;
E-mail: sunil20051@rediffmail.com
Prakash C. Supakar, Ph.D,
Institute of Life Sciences, Nalco Square,
Bhubaneswar-751023, India,
Phone: 91-674-2302783;
Fax: 91-674-2300728;
E-mail: pcsupakar@hotmail.com |
|
| Received July 23, 2008; Accepted August 25, 2008; Published September 13, 2008 |
| Citation:Sunil K, Priya RD, Prakash CS (2008) Prediction of 3-Dimensional Structure of Cathepsin L Protein of Rattus
Norvegicus. J Proteomics Bioinform 1: 307-314. doi:10.4172/jpb.1000039 |
| Copyright: © 2008 Sunil K, etal. This is an open-access article distributed under the terms of the Creative Commons
Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original
author and source are credited. |
| Abstract |
Cathepsin L is a cysteine protease which degrades connective tissue proteins like collagen, elastin and
fibronectin. Increase in the expression of cathepsin L in aged kidney leading to considerable loss of organ
function in old age. Recently it has been reported that SARS-CoV or SARS-CoV spike protein-pseudotyped
retroviruses utilize the enzymatic activity of endosomal cathepsin L protease for viral entry. A 3D structure of rat
cathepsin L was constructed in this report through homology modeling using the X-ray structure of procathepsin
L from Homo sapiens (PDB code: 1CS8). The homology modeling was done by using the MODELLER 9v2
software. The final model obtained by molecular mechanics and dynamics method and was assessed by
PROCHECK and VERIFY 3D graph, which showed that the final refined model is reliable. The model could be
further explored for characterizing the protein. |
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