Range Charts for Agreement in Measurement Comparison Studies, With Application
to Replicate Mass Spectrometry Experiments |
| James A. Koziol1, Anne C. Feng1,Jingyi Yu2, Noelle M. Griffin2, Jan E. Schnitzer2 |
1Department of Molecular and Experimental Medicine, The Scripps Research Institute,
10550 North Torrey Pines Road, La Jolla, CA 92037 |
| 2Sidney Kimmel Cancer Center, 10905 Road to the Cure, San Diego, CA 92122 |
| Corresponding author: |
James A. Koziol, Ph.D, Department of Molecular and Experimental Medicine, MEM216
The Scripps Research Institute, 10550 North Torrey Pines Road,
La Jolla, CA 92037,
Fax: 858-784-2664,
E-mail:koziol@scripps.edu, 858-784-2703 |
|
| Received August 02, 2008; Accepted September 02, 2008; Published September 13, 2008 |
| Citation:James AK, Anne CF, Jingyi Y, Noelle MG, Jan ES (2008) Range Charts for Agreement in Measurement Comparison Studies,
With Application to Replicate Mass Spectrometry Experiments. J Proteomics Bioinform 1: 287-292. doi:10.4172/jpb.1000036 |
Copyright: © 2008 James AK, etal. This is an open-access article distributed under the terms of the Creative Commons
Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original
author and source are credited. |
| Abstract |
It is important to investigate the reproducibility of raw mass spectrometry (MS) features of abundance, such
as spectral count, peptide number and ion intensity values, when conducting replicate mass spectrometry
measurements. Reproducibility can be inferred from these replicate data either formally with analyses of variance
techniques or informally with graphical procedures, particularly, Bland-Altman plots on paired runs. In this
note, we suggest range plots to provide a suitable generalization of Bland-Altman plots to experiments with
more than two replicate runs. We describe range charts and their interpretation, and illustrate their use with data
from a recent proteomic study relating to label-free analysis. |
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