In Silico Prediction of the Tertiary Structure of M. leprae
Hsp65 Protein Shows an Unusual Structure in Carboxy-terminal Region |
Rossetti RAM1,2, Lorenzi JCC1, Giuliatti S1, Silva CL1, Coelho-Castelo AAM1 |
| 1Department of Biochemistry and Immunology, School of Medicine of
Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil |
| 2Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, University of São Paulo , Brazil |
| Corresponding author: |
Dr. Coelho-Castelo AAM, Departamento de Bioquimica e Imunologia, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Av. Bandeirantes, 3900, 14049-900, Ribeirão Preto, SP,
Phone: 55-16-36024532 / 36024538,
E-mail: arlete@fmrp.usp.br |
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| Received December 14, 2008; Accepted December 24, 2008; Published December 26, 2008 |
| Citation: Rossetti RAM, Lorenzi JCC, Giuliatti S, Silva CL, Coelho C AAM (2008) In Silico Prediction of the Tertiary Structure of M. leprae Hsp65 Protein Shows an Unusual Structure in Carboxy-terminal Region. J Comput Sci Syst Biol 1: 126-131. doi:10.4172/jcsb.1000012 |
| Copyright: ©2008 Rossetti RAM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| Abstract |
DNA vaccines have been used with great success in experimental and some clinical therapy. However, the
mechanisms of activation of the immune system by these vaccines are not utterly understood yet. Hsp65 is a Mycobacterium leprae chaperone whose gene has been efficiently used as experimental DNA vaccine against
tuberculosis and clinical trial against tumor. Since little is know about the three-dimensional (3D) structure of
hsp65 and modeling of 3D protein structure can increase the information to improve the knowledge about the mechanism action as well as the design of new DNA vaccine formulation, here we used the bioinformatics to get the
design in silico of hsp65 (heat shock protein) molecule. The determination of hsp65 3D structure was obtained
by homology using the software Modeller (Eswar et al., 2001). It was used two proteins as models: 1SJP, a 60-
kDa chaperonin from Mycobacterium tuberculosis in the PDB, and the 1WE3, the crystal structure of the chaperonin
complex Cpn60/Cpn10/(ADP)7 from Thermus thermophilus). Our results showed an interesting structure in
Hsp65 that could be important in development or modulation of immune response. |
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