Figure 1: (A) Schematic of water/oil/water(W/O/W) double-emulsion microparticle synthesis technique. (B) Confocal image of FITC-albumin loaded high molecular weight PLGA microspheres at ultrasonication power setting #1 with 40x objective. Scale bar = 20µm. (C) Microparticle size when synthesized with low and high molecular weight (LMW and HMW, respectively) PLGA at ultrasonication power settings #1, 3, and 5. (** = p<0.05 compared to power #1). Decreases in polymer size and ultrasonication power decreases microparticle size. (D) 3-D Confocal projection of FITC-albumin loaded PLGA microsphere at day 0 (top) and day 14 (bottom). Note PLGA degradation and loss of drug at day 14. Image obtained at 63x under oil. Scale bar = 10µm.